4种中药多糖对猪脾淋巴细胞增殖的影响  

Effects of four Chinese herbal medicinal polysaccharides on proliferation of swine splenic lymphocytes

作　　者：赵现敏[1] 崔保安[1] 张红英[1] 胡梅[1] 王远阁[1] 李改英[1] 刘炜 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]郑州市畜牧局,河南郑州450002

出　　处：《河北农业大学学报》2007年第3期82-85,共4页Journal of Hebei Agricultural University

基　　金：国家"十五"食品安全重大攻关专项(2001BA804A30-11);河南省杰出人才创新基金(0621002100)

摘　　要：为筛选免疫活性较好的中药多糖和研制新型猪用免疫增强剂,用MTT比色法比较了不同浓度的板蓝根多糖(IRPS)、牛膝多糖(ABPS)、山药多糖(CYPS)和黄芪多糖(APS)等4种中药多糖对猪脾脏淋巴细胞增殖的影响。结果表明,IRPS,ABPS,CYPS,APS均能显著刺激ConA或LPS诱导的猪脾淋巴细胞增殖(P<0.05),且4种多糖促进猪脾淋巴细胞增殖的作用与多糖浓度有关。In order to select Chinese herbal medicinal polysaccharide （CHMP） that has immunocompetence and develop a new-type immunopotentiator used by swine, effects of four Chinese herbal medicinal polysaccharides on proliferation of swine splenic lymphocytes were compared by the methods of MTT colorimetric assay. The four CHMPs including Isatis root polysaccharide （IRPS）, Achyranthes bidentata polysaccharide （ABPS）, Chinese yam polysaccharide （CYPS） and Astragalus polysaccharide （APS） were chosen for the experiment. The results showed that IRPS, ABPS, CYPS and APS could promote proliferation of swine splenic lymphocytes significantly（P 〈0.05）, which were induced by ConA or LPS, and the effects were related to concentration of polysaccharides.

关 键 词：中药多糖 免疫增强剂 猪 脾脏淋巴细胞 MTT比色法 

分 类 号：S853.74[农业科学—临床兽医学]