肝炎灵等中药针剂低分子多糖的紫外分光光度法检测  

Determining content of lower-molecular-weight polysaccharides from some herbal injections including Gan Yan Lin with ultra-spectrophotometer

作　　者：苏毅[1] 王燕[2] 金树根[2] 邓雯 李兆健[4] 王庆其[4] 

机构地区：[1]浙江省台州市玉环县中医院,317600 [2]上海中医药大学附属曙光医院,201203 [3]江西省丸江学院检验系04级,332000 [4]上海中医药大学,201203

出　　处：《中国医药指南（学术版）》2007年第11S期348-351,共4页Guide of China Medicine

基　　金：上海市教委高校高水平特色发展项目：沪教委财（2005）81号“中医经典防治重大疫病的继承与发展研究”子课题：“山豆根注射液肝炎灵抗HBV活性有效部位筛选和作用机制初步研究”

摘　　要：采用正丁醇萃取，以葡萄糖为标准品，加入硫酸反应各小时后于247nm处读取吸光度的紫外分光光度法，检测肝炎灵、红花、茵栀黄、清开灵、丹参、黄芪等几种中药注射制剂中低分子多糖含量，发现上述中药注射制剂低分子多糖含量（由高至低）为：肝炎灵〉红花〉茵栀黄〉清开灵〉丹参〉黄芪，此实验结果上述中药注射制剂的免疫调节活性的药理作用机制提供实验依据。The study was aimed to measure the contents of lower-molecular-weight polysaccharides in some kind of herbal injections such as Gan Yah Lin（Radix sophorae tonkinensis）,Hong Hua（Flower carthamus tinctorirs）,Yin Zhi Huang,Qing Kal Ling, Dan Shen （Radix salviae miltiorrhizae）,Huang Qi （Radix astraagalus membranceus） with method of ultra-spectrophotometer extracted by n-butanol and glucose as standard, it was found that the contents of lower-molecular-weight polysaccharides in those injections were in the followed order（from high to low） Gan Yan Lin, Hong Hua, Yin Zhi Huang, Qing Kal Ling, Dan Shen and Huang Qi.The study provides the evidence that the above injections have an immunological regulatory effect.

关 键 词：肝炎灵 红花 茵栀黄 清开灵 丹参 黄芪多糖 多聚糖 低分子多糖 低聚糖 寡糖 寡聚糖 

分 类 号：R969[医药卫生—药理学]