川芎嗪对哮喘不同时段肺组织α-SMA干预机制的实验研究  

Investigation of effect of Ligustrazine on expression of α-smooth muscle actin in lung tissue in allergy-induced rat asthmatic model

作　　者：李羚[1] 杨莉[2] 唐珩[2] 金蕊[2] 

机构地区：[1]南京医科大学附属无锡市儿童医院呼吸科,江苏无锡214002 [2]东南大学附属中大医院儿科,江苏南京210009

出　　处：《中国现代医学杂志》2008年第11期1548-1551,共4页China Journal of Modern Medicine

摘　　要：目的探讨不同时段大鼠哮喘模型肺组织α-平滑肌肌动蛋白(alpha-smooth muscle actin,α-SMA)在转录和蛋白水平的表达及川芎嗪对其的作用机制。方法以卵蛋白致敏制备大鼠哮喘模型,腹腔注射不同剂量川芎嗪、地塞米松及联合用药(川芎嗪+地塞米松)干预,分别在激发干预后的1周、2周取肺组织,采用逆转录聚合酶链反应(semi-quantitatively reverse transcription-polymerase chain reaction,RT-PCR)和免疫组织化学S-P方法研究肺组织α-SMA的表达。结果与正常组比较,在激发哮喘后1周肺组织α-SMA的表达显著升高(P<0.05),2周后表达更加显著(P<0.001);与哮喘模型组比较,各用药干预组α-SMA的表达均显著降低(P<0.001),且随着干预时间的延长降低幅度增大,川芎嗪大剂量干预组与地塞米松组相似,干预至两周时接近正常水平,二者降低的幅度均大于川芎嗪小剂量和联合干预组,联合用药干预组的效果优于川芎嗪小剂量组。结论哮喘肺组织α-SMA过量表达与哮喘的发生发展密切相关,并在早期即开始参与哮喘的发病。川芎嗪可在转录和蛋白水平抑制哮喘大鼠肺组织α-SMA的过度表达。[Objective] To observe the effect of Ligustrazine on expression of α-SMA in the lung tissue of rats with asthma. [Methods] Sprague-Dawley（SD） rats were randomly divided into 6 experimental groups： normal group （A）, model group（B）, Dexamethasone group（C）, low-dose of Ligustrazine group（D）, high-dose of Lignstrazine group （E）, combinative therapy group（F）. The asthmatic model was established by repeated inhalation of Gvalbulium. The expression of α-SMA in the lung tissue of rats was detected by semi-quantitatively reverse transctiption-polymerase chain reaction （RT-PCR） and means of immunohistochemical staining. [Results] There was little expression of α- SMA in the lung tissue of normal rats. The expressions of α-SMA increased 1 week after OVA challenge （P 〈0.001） and increased more significantly 2 weeks after OVA challenge compared with that in group A and the same group in 1 week （P 〈0.001）. In group C, D, E and F, the contents of α-SMA were significantly lower than that in group B （P 〈0.001）. After intervening 2 weeks the contents of α-SMA in group C and group E were approaching that in the normal group and lower than that in group D and group F. The effect in group F was better than that in group D. [Conclusion] Overexpression of α-SMA in asthmatic lung tissue is closely related to the onset and development of asthmatic. Ligustrazine could inhibit the development of asthma by the way of suppressing the expression of α-SMA in the levels of transcription and protein.

关 键 词：哮喘 α-平滑肌肌动蛋白(α-SMA) 川芎嗪 

分 类 号：R562.25[医药卫生—呼吸系统]