多倍体萱草的组织培养与快速繁殖技术  

Tissue Culture and Rapid Propagation of Hemerocallis hybrida

作　　者：袁爱民[1] 佟宝君[2] 郝砚英 柴洁婷[2] 

机构地区：[1]天津红港绿茵花草有限公司,天津300402 [2]天津市园林局天津花圃,天津300112

出　　处：《天津农业科学》2008年第3期45-47,共3页Tianjin Agricultural Sciences

摘　　要：以多倍体萱草的芽和花蕾为外植体,以MS为基本培养基,进行组织培养与快速繁殖的研究。结果表明,MS+6-BA 2.0 mg/L+NAA 0.2 mg/L为最佳诱导培养基,MS+6-BA 0.5 mg/L+NAA 0.01 mg/L为较好的增殖培养基。1/2 MS+NAA0.02 mg/L为较好的生根培养基,用腐叶土和珍珠岩4∶1比例做基质移栽苗成活率可达80%以上。Tissue culture and propagation were studied by taking HemerocaUis hybrida as explant and using MS as basic medium, the results indicated that the induction medium with MS＋6-BA2.0 mg/L＋NAA0.2 mg/L was the best, and the medium with MS＋6- BA0.5 mg/L＋NAA0.01 mg/L was better multiplication medium. The medium with 1/2 MS＋NAA0.02 mg/L was better for rooting. The survival rate of transplanted plantlets on media which contained band and perlite with 4：1 was more than 80%.

关 键 词：多倍体萱草 组织培养 快速繁殖 

分 类 号：S682.19[农业科学—观赏园艺]