全血及血浆病毒分离方法在SHIV-KB9感染猴模型中的应用  

Virus Isolation From SHIV-KB9-infected Rhesus Monkeys

作　　者：丛喆[1] 王卫[1] 许琰[1] 蒋虹[1] 杨贵波 魏强[1] 

机构地区：[1]中国医学科学院中国协和医科大学实验动物研究所,北京100021 [2]中国性病艾滋病预防控制中心中国CDC疾病预防控制中心,北京100050

出　　处：《医学动物防制》2008年第6期401-403,共3页Journal of Medical Pest Control

基　　金：中国CIPRA项目;美国NIH资助;项目号U19AI51915-04

摘　　要：目的使用全血和血浆病毒分离方法分离SHIV-KB9感染标本,验证方法的可行性和敏感性。方法将SHIV-KB9感染恒河猴的血浆和全血标本与CEMx174细胞共培养,观察细胞病变,测定感染猴血浆病毒载量。结果全血病毒分离的滴度与血浆病毒载量测定结果趋势相符,高峰相同,但血浆病毒分离阳性分离率较低。结论全血病毒分离敏感性高于血浆病毒分离,可用于SHIV-KB9感染猴模型。Objective Isolated the SHIV - KID from the whole blood and plasma of SHIV - KID infected monkeys, to test the sensibility and specificity of these two methods. Methods Cocultured the plasma and the whole blood from SHIV - KB9 infected monkeys, observed the CPE and quantified the viral load in plasma. Results Virus isolation from whole blood was parallel with viral load in plasma. The positive rate from plasma was lower than whole blood. Conclu- sion It has higher sensitivity, specificity and stability to isolate SHIV - KID from whole blood than plasma so that it could be used for the evaluation of SHIV - KB9 infected monkey model.

关 键 词：SHIV 病毒分离 病毒载量 

分 类 号：R593.31[医药卫生—内科学]