p，p’-DDE、β-BHC对大鼠支持细胞FasL mRNA表达及NF-κB活性的影响  

Combined Effects of p,p'-DDE andβ-BHC on the Expression of FasL mRNA and Activation of NF-κB in Rat Sertoli Cells

作　　者：王轶楠[1] 于海歌[1] 宋杨[1] 胡雅飞[1] 梁先敏[1] 居颖[1] 杨克敌[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系、环境与健康教育部重点实验室,湖北武汉430030

出　　处：《环境与职业医学》2008年第3期248-251,共4页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金项目(编号：30671734)

摘　　要：[目的]研究有机氯农药DDT和六六六的代谢产物(p,p’-DDE)和β-BHC及联合染毒对离体培养大鼠支持细胞(sertoli cell)FasL mRNA表达及NF-κB活性的影响。[方法]分离大鼠睾丸支持细胞进行原代培养2d,以DMSO为溶剂对照,分别以终浓度为10、30、50μmol/L的p,p’-DDE、β-BHC及联合染毒支持细胞24h,应用RT-PCR法研究支持细胞FasL mRNA的表达;用激光共聚焦显微镜检测NF-κB的转位激活状况。[结果]10、30、50μmol/L的p,p’-DDE、β-BHC及其联合染毒支持细胞后,FasL mRNA表达升高,50μmol/L剂量组与对照组差异有统计学意义(P<0.05),各组内低、中剂量组与高剂量组差异有统计学意义(P<0.05);NF-κB活性明显增强。[结论]支持细胞经p,p’-DDE、β-BHC及其联合染毒后,FasL mRNA表达明显升高,NF-κB的活性随染毒剂量的增加而增强,且联合作用比单独作用更加明显。[ Objective ] To study the combined effects of p, p＇-DDE and β-BHC on the expression of FasL mRNA and activation of NF-κB of rat Sertoli cell in vitro. [ Methods ] Sertoli ceils after separating from testicular tissue of SD rats were cultured for 2d, then incubated with DMSO as control group, also incubated with p, p＇-DDE, β-BHC and their combination as test groups in a series of concentrations （ 10, 30, 50 μmol/L ） respectively in vitro for 24h. The FasLmRNA expression was detected by two-step RT-PCR; NF-κB activation state was examined under laser confocus microscope. [ Results ] The expression of FasL mRNA was increased with the increasing of toxicant concentrations in cell culture. There was statistical significance between the concentrations of 50μmol/L and control group separately （ P 〈 0.05 ）, there was also statistical significance between the concentrations of 50 μmol/L and 10, 30 μmol/L separately （P 〈 0.05 ）. The transposition of NF-κB was obviously increased. [ Conclusion ] The results suggest that the expression of FasL mRNA can be enhanced by p, p＇-DDE, β-BHC and their combination, the activation of NF-κB can be enhanced with the increase of concentration of p, p＇-DDE, β-BHC and their combination. The combined effects of p, p＇-DDE and β-BHC on Sertoli ceils were more obvious.

关 键 词：P p'-DDE β—BHC FASL NF-ΚB 支持细胞 

分 类 号：R114[医药卫生—卫生毒理学]