纳米二氧化钛对氯化镉所致人胚肝L-02细胞毒性作用的影响  

Influence of nano-TiO_2 on the Toxic Effects of Cadmium Chloride in Human Embryo Liver L-02 Cells

作　　者：高霞[1] 张娜[1] 杜宏[1] 张江华[1] 江明[2] 吕斌[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院教育部环境与健康重点实验室,湖北武汉430030 [2]华中科技大学同济药学院,湖北武汉430030

出　　处：《环境与职业医学》2008年第3期256-258,共3页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金项目(编号:20407010);教育部新世纪优秀人才支助计划(编号:NCET-06-0640)

摘　　要：[目的]研究纳米二氧化钛(nano-TiO_2)对氯化镉(CdCl_2)所致人胚肝细胞毒性作用的影响。[方法]不同浓度的CdCl_2(0.001、0.01、0.1μmol/L)单独或与0.01 mg/L nano-TiO_2混合后,分别作用于人胚肝L-02细胞24h,观察各组L-02细胞的DNA损伤水平,以及hMsh2基因(hMsh2)、O^6-甲基鸟嘌呤甲基转移酶(MGMT)和DNA依赖蛋白激酶复合物催化亚基(DNA-PKcs)的mRNA表达水平。[结果]与相应剂量的CdCl_2单独染毒组比较,nano-TiO_2与各浓度的CdCl_2混合染毒组L-02细胞的DNA损伤加重(P<0.05),hMsh2表达水平明显上升(P<0.05),MGMT表达水平无显著性变化,nano-TiO_2与0.01、0.1μmol/L的CdCl_2混合染毒组DNA-PKcs的基因表达水平明显上升(P<0.05)。[结论]0.01mg/L的nano-TiO_2可以加重各浓度CdCl_2对L-02细胞的DNA单链损伤,从而使CdCl_2对L-02细胞毒性作用增强。[ Objective ] To investigate the influence of nano-TiO2 on the toxic effects of cadmium chloride in human embryo liver L-02 cell line. [ Methods ] L-02 cells were exposed to different concentration of cadmium chloride（ 0.001,0.01, 0.1 μmol/L ）, or different concentration of cadmium chloride ＋ nano-TiO2 （ 0.01 mg/L ） respectively for 24 hours. DNA damage level was detected by comet assay. The expression level of DNA repair enzymes O^6-methylguanine-DNA methyltransferase （ MGMT ）, DNA-dependent protein kinase catalytic subunit （ DNA-PKcs ） and hMsh2 were measured by RT-PCR. [ Results ] Compared with the corresponding cadmium chloride exposed groups, 0.001,0.01, 0.1 μmol/L cadmium chloride ＋ nano-TiO2（ 0.01 μmol/L ） increased DNA damage level and the expression level of hMsh2 respectively （ P 〈 0.05 ）; while 0.01, 0.1 μmol/L cadmium chloride ＋ nano-TiO2 （ 0.01 mg/L ）increased the expression level of DNA-PKcs respectively （ P 〈 0.05 ）, but no significant effect on the expression level of MGMT was observed. [ Conclusion ] 0.01 mg/L nano-TiO2 could aggravate the toxicity induced by cadmium chloride in L-02 cell line.

关 键 词：彗星试验 氯化镉 纳米二氧化钛 DNA损伤 DNA修复酶 L-02细胞 

分 类 号：R114[医药卫生—卫生毒理学]