落新妇根药材中岩白菜素定性定量方法研究  

Methodology Study on Qualitative and Quantitative Analysis of Bergeninum in Chinese Astilbe Rhizome

作　　者：来平凡[1] 蒋士鹏[1] 杨一令[1] 吴人杰 

机构地区：[1]浙江中医药大学,杭州310053 [2]湖州市药品检验所,浙江湖州313000

出　　处：《中国现代应用药学》2008年第3期200-202,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省教育厅资助项目(961122)

摘　　要：目的对落新妇根中岩白菜素的鉴别及含量测定方法进行研究。方法采用薄层色谱法对落新妇根中的岩白菜素进行鉴别，采用高效液相色谱法测定落新妇根中岩白菜素的含量。结果薄层斑点清晰，重现性好；岩白菜素选定浓度0．025-0．25mg·mL^-1内线性关系良好，回归方程为：Y=28159X＋50585，r=0．9999，回收率为99．16％（RSD为2．0％）。结论定性鉴别简便快速，专属性强；定量分析灵敏准确、重复性好，为落新妇根的质控提供了可靠的方法。OBJECTIVE To study the identification and determination of Bergeninum in Chinese astilbe rhizome. METHODS Bergeninum in Chinese astilbe rhizome was identified by TLC, and the content of Bergeninum was determined by HPLC. RESULTS Sopts were clear with good recurrence; The linear range of Bergeninum was 0. 025 -0.25 mg · mL^- 1 , and the regression equation was Y = 28 159X ＋50 585 ,r =0. 999 9 with the recovery 99.16% （ RSD 2.0% ）. CONCLUSION The study offered a credible method for quality of Chinese astilbe rhizome. The qualitative identification method was simple and effective with high specificity. The quantitative analysis method was sensitive, exact and had good recurrence.

关 键 词：落新妇根 岩白菜素 薄层色谱法 高效液相色谱法 

分 类 号：R931.6[医药卫生—生药学]