金属蛋白酶解离素28对人牙乳头细胞生物学特性的影响  

The effects of ADAM28 on HDPC biological characteristics

作　　者：赵征 徐军明[2] 赵威丽[2] 

机构地区：[1]解放军济南军区第401医院口腔科,山东青岛266071 [2]济南军区青岛第二疗养院,山东青岛266071

出　　处：《上海口腔医学》2008年第3期280-284,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30572046)~~

摘　　要：目的:探讨金属蛋白酶解离素28(ADAM28)基因对人牙乳头细胞(HDPC)增殖、分化、凋亡的影响,并分析其可能的作用机制。方法:应用细胞培养、基因转染、MTT、流式细胞术(FCM)和酶动力学等方法,探讨ADAM28基因对HDPC生物学特性的影响,采用SPSS12.0软件包中的SNK检验进行统计学分析。结果:成功转染ADAM28真核质粒,真核质粒转染组HDPC的增殖活性、增殖指数、碱性磷酸酶(ALP)分泌活性显著高于空载体组、未转染组,而凋亡细胞百分比显著低于两对照组,P<0.01。结论:ADAM28可显著促进HDPC的增殖和ALP的分泌,显著抑制HDPC的凋亡。PURPOSE： To study the effects of ADAM28 gene on the proliferation, differentiation and apoptosis of human dental papilla cells（HDPC） and analyze the possible mechanism. METHODS： Cell culture, gene transfeetion, MTT, flow eytometry （FCM）and enzyme kinetics method were used to study the effects of ADAM28 on HDPC biological characteristics. SNK test of SPSS12.0 software package was used for statistical analysis. RESULTS： ADAM28 eukaryotie plasmid was transfected into HDPC successfully, HDPC proliferation activity, proliferation index（PI） and ALP secretion activity in eukaryotic plasmid group were significantly higher than those in pcDNA3.1（＋） group, untransfeeted group, and apoptosis cell percentage in eukaryotic plasmid group was lower than that in the control groups.There were significant differences between them （P〈0.01）. CONCLUSION： ADAM28 could significantly promote the proliferation and ALP secretion of HDPC, inhibit HDPC apoptosis. Supported by National Natural Science Foundation of China （Grant No. 30572046）.

关 键 词：金属蛋白酶解离素28 人牙乳头细胞 增殖 分化 凋亡 

分 类 号：R78[医药卫生—口腔医学]