nHACP/CF材料与成骨细胞体外复合培养的实验研究  

Study of nHACP/CF co-cultured with osteoblasts in vitro

作　　者：孙宁宁[1] 王绪凯[1] 周青[1] 卢利[1] 黄绍辉[1] 

机构地区：[1]中国医科大学口腔医学院口腔颌面外科,辽宁沈阳110002

出　　处：《上海口腔医学》2008年第3期313-316,共4页Shanghai Journal of Stomatology

摘　　要：目的:检测甲壳素纤维增强多孔胶原基骨组织工程支架材料(nHACP/CF)对成骨细胞附着增殖的影响,为骨组织工程支架材料的选择提供实验依据。方法:原代培养大鼠成骨细胞,将第3代细胞与nHACP/CF材料体外复合培养。碱性磷酸酶(ALP)染色对成骨细胞进行鉴定;将不同浓度成骨细胞接种到nHACP/CF支架上,通过检测ALP活性测定最佳接种浓度;通过扫描电镜观察和各培养时点细胞增殖率及ALP活性检测,评价nHACP/CF材料对成骨细胞的影响。采用SPSS11.5软件包对测定结果进行单因素方差分析。结果:大鼠成骨细胞ALP染色阳性。统计分析表明,6×105/ml为细胞最佳接种浓度;大鼠成骨细胞能在nHACP/CF材料上附着、增殖,其生长及功能不受抑制。结论:nHACP/CF材料是大鼠成骨细胞附着生长的良好载体。PURPOSE： To investigate the effect of nHACP/CF on coherence and proliferation of osteoblast. METHODS： The rat osteoblasts were isolated and cultured, then co-cultured with nHACP/CF in vitro. The cells were observed under cytochemical staining for ALP; the cell-material complex was observed under electronic scanning microscope, MTT was used for detection of cell proliferation,and ALP activity was determined at different times.SPSS 11.5 software package was used for one-way ANOVA. RESULTS： Cytochemical staining for ALP was positive. The best density of seeding cells was 6×10^5/ml; osteoblast could attach and extend on nHACP/CF,the proliferation and growth were not affected by nHACP/CF. CONCLUSION： nHACP/CF is a good vehicle for rat osteoblast to grow on.

关 键 词：骨组织工程 纳米羟基磷灰石 支架材料 成骨细胞 

分 类 号：R318.08[医药卫生—生物医学工程]