HSVⅠ感染L-02细胞对核不均一性核糖核蛋白H2(hnRNP H2)表达影响  

The Effect of HSVⅠ Infection on the Expression of hnRNP H2 in Human Fetal Liver Cell

作　　者：王赛锋[1] 寸韡[1] 董承红[1] 洪敏[1] 刘龙丁[1] 王蕾[1] 李琦涵[1] 

机构地区：[1]中国医学科学院/北京协和医学院医学生物学研究所,昆明650118

出　　处：《中国生物工程杂志》2008年第6期18-22,共5页China Biotechnology

基　　金：国家自然科学基金资助项目(30570081,30570094)

摘　　要：单纯疱疹病毒(HSVⅠ)对宿主细胞转录及mRNA修饰翻译通路的调控是一个系统的、复杂的体系,探索其具体机制将有助于了解病毒复制过程及与宿主细胞之间的相互作用。基于2-DE分析,人正常肝细胞L-02细胞核不均一性核糖核蛋白H2(heterogeneous nuclear ribonucleoprotein H2,hnRNP H2)在感染HSVⅠ前后存在表达差异,通过Western blot,Northern blot等技术方法验证其在病毒复制过程中不同时段对其表达影响。Abstract in host cell is infection will a Herpes simplex virus I （ HSV Ⅰ ） regulating the pathway of transcription and translation modify very systematic and complicate system. A clear understanding of the concrete mechanisms greatly help to comprehend the virus replication and the interaction with the host cell. By of the analysis of 2-DE, the heterogeneous nuclear ribonucleoprotein H2 in human fetal liver cell represent distinction after the HSV I infection. Utilization of Northern blot and Western blot technologies verified the expression of hnRNP H2 in different stage of virus infection is varied.

关 键 词：HSVⅠ 核不均一性核糖核蛋白H2(hnRNP H2) L-02 Northern BLOT 

分 类 号：Q756[生物学—分子生物学]