克雷伯杆菌诱变菌株利用生物柴油副产物甘油制备3-羟基丙醛  

3-HPA Production by Mutant Klebsiella penumonia Using Biodiesel Waste Glycerol

作　　者：彭益强[1] 林芳[1] 王园园[1] 方柏山[1] 张文珍[2] 

机构地区：[1]华侨大学工业生物技术福建省高等学校重点实验室,福建泉州362021 [2]华侨大学信息科学与工程学院,福建泉州362021

出　　处：《激光生物学报》2008年第3期344-348,362,共6页Acta Laser Biology Sinica

基　　金：国家"863计划"子课题(2006AA020103);国家自然科学基金项目(20676048);国务院侨办课题(06QZR08)

摘　　要：应用克雷伯杆菌诱变菌株(Kp8)分别在游离与固定化态下转化生物柴油副产物甘油(粗甘油)制备3-羟基丙醛(3-HPA)。结果发现,Kp8在含30 g/L粗甘油发酵培养基(实验条件I)中较初始克雷伯杆菌(Kp0)在含30 g/L纯甘油培养基(实验条件II)中发酵产3-HPA的相对产率提高了13.75%;Kp8与Kp0经溶胶-凝胶法固定后分别在实验条件I、II下发酵,前者3-HPA相对产率为游离Kp0的73.75%,后者为65.6%;固定化细胞分别进行了7个批次的发酵,实验条件I中3-HPA相对产率由80%降至60%,3-HPA总得率从0.321 g/g降到0.243 g/g,3-HPA转化率从40.1%降到30.4%,实验条件II中3-HPA相对产率维持在70%~80%水平,3-HPA总得率维持在0.315 g/g^0.276 g/g,3-HPA转化率维持在39.4%~34.5%。Mutant Klebsiella penumonia （ Kp8 ） transform biodiesel waste glycerol to 3-HPA under free and immbolization condition. It showed that 3-HPA relative production rate of Kp8 conversion 30 g/L waste glycerol （ condition Ⅰ） was 13.75 % higher than originality Klebsiella penumonia （Kp0） conversion 30 g/L simple glycerol （condition Ⅱ） ; Kp8 and Kp0 immobilized by sol-gel and ferment under condition Ⅰ and condition Ⅱ, 3-HPA relative producing rate of former was 73.75 % of free Kp0＇s, the latter was 65.6 % ; Immobilized cells fermented for 7 batchs, 3-HPA producing rate of condition Ⅰ fell from 80 % to 60 % , 3-HPA overall yield fell from 0. 321 g/g to 0. 243 g/g, conversion rate was fall from 40.1% to 30.4 % , 3-HPA relativity produce rate of condition Ⅱ remained between 70 % - 80 % , 3-HPA overall yield remained between 0. 315 g/g -0. 276 g/g, conversion rate remained between 39.4 % - 34.5 % .

关 键 词：3-羟基丙醛 克雷伯杆菌 溶胶-凝胶固定化 甘油 

分 类 号：Q643[生物学—生物物理学] Q55