中华大蟾蜍和花背蟾蜍染色体同源性研究  

CHROMOSOME HOMOLOGY BETWEEN  Bufo bufo gargarizans AND  Bufo raddei

作　　者：王亚军[1] 王晓飞[1] 王喜忠[1] 李娟[1] 王子淑[1] 陈文元[1] 

机构地区：[1]四川大学生物学系

出　　处：《Zoological Research》1997年第4期415-419,共5页动物学研究（英文）

基　　金：国家自然科学基金

摘　　要：本文以培养的中华大蟾蜍（Ｂｕｆｏｂｕｆｏｇａｒｇａｒｉｚａｎｓ）、花背蟾蜍（Ｂｕｆｏｒａｄｄｅｉ）的外周血淋巴细胞为实验材料，采用ＢｒｄＵ标记法和胸苷标记的方法，获得了中华大蟾蜍与花背蟾蜍清晰的高分辨晚复制带，确定了中华大蟾蜍每一染色体的特征性晚复制区；并就中华大蟾蜍与花背蟾蜍的晚复制带纹进行了精确比较，发现中华大蟾蜍与花背蟾蜍的染色体之间具极高同源性。该结果说明：在相同的培养条件下，只要ＢｒｄＵ掺入Ｓ期的时间较为统一，显示复制带的条件基本一致的情况下。High-resolution late replication banding patterns were obtained on prometaphase chromosomes of Bufo bufo gargarizans and Bufo raddeiby treating cultured lymphocytes with 5-bromodeoxyuridine (BrdU) The characteristic late replication regions on each banded chromosome of Bufo bufo gargarizans, which can be easily distinguished from other chromosomes, have been observed Precise comparison between two species′ highresolution late replication banding patterns indicates that all chromosomes of two species are highly homologous These results show that under the same culture condition and at the same time when BrdU were incorporated into DNA, it is possible to research chromosome homology among different species by comparing replication banding patterns

关 键 词：中华大蟾蜍 花背蟾蜍 染色体 同源性 

分 类 号：Q959.530.2[生物学—动物学]