乙型肝炎病毒表面抗原中蛋白基因在昆虫细胞中的表达及其初步鉴定  

Expression of HBV Surface Antigen Middle Protein Gene in Insect Cells and Preliminary Detection of Biological Activity of Expressed Products

作　　者：尚继军[1] 项金忠[1] 谢云[1] 张国强 冯素英 张鹏飞[1] 

机构地区：[1]卫生部北京生物制品研究所,北京100024

出　　处：《中国生物制品学杂志》1997年第4期196-199,共4页Chinese Journal of Biologicals

摘　　要：从乙肝阳性人血清中克隆HBV含前s区表面抗原基因（PreSZ＋S），将其插入到杆状病毒转移质粒PVL1393中，并与昆虫病毒ACNPVDNA共转染Sf9昆虫细胞，筛选出重组病毒。用乙肝放免（RIA）试剂盒及EIAPreS2－Ag检测试剂盒检测重组病毒感染的Sf9细胞，表明细胞中有HBsAg及PreS2－Ag存在。将重组病毒感染的细胞免疫小鼠，在小鼠血清中能检测到anti-HBsAg及anti-PreS2抗体。HBV DNA was extracted from HBsAg positive serum and used for the amplication of the whole gene coding HBV surface antigen middle protein by PCR. The gene was inserted into a baculovirus transfer vector pVL1393 and cotransfected into Sf9 insect cells with wild AcNPV DNA,then recombinant virus was screened out. By using RIA and EIA kits, HBsAg and PreS2-Ag were detected out in Sf9 cells infected with the recombinant virus,and the corresponding antibodies of them were also detected out in the serum of mice imunized with the cells.

关 键 词：乙型肝炎病毒 表面抗原 昆虫细胞 蛋白基因 

分 类 号：R512.620.3[医药卫生—内科学] R392.11[医药卫生—临床医学]