两种微藻凝集素对几种不同类型细胞的凝集作用  

Agglutination of different cells by two lectins from microalgae

作　　者：郑怡[1] 陈晓清[2] 林雄平 

机构地区：[1]发育与神经生物学福建省高校重点实验室,福建师范大学生命科学学院,福建福州350108 [2]漳州师范学院生物科学与技术系,福建漳州363000 [3]宁德高等师范专科学校生物系,福建宁德352100

出　　处：《海洋科学》2008年第6期30-34,共5页Marine Sciences

基　　金：福建省科技计划重点项目(2005N036);福建省自然科学基金项目(B0510008);福建省科技厅项目(K04024)

摘　　要：经离子交换和分子筛层析从紫球藻(Porphyridium cruentum)和海水小球藻(Chlorellasp.)中分离纯化得到2种凝集素,研究它们对几种不同类型细胞即动物和人血红细胞、海洋和淡水微藻、细菌和真菌的凝集活性。研究结果表明,2种微藻凝集素对它们当中一些类型的细胞或种类具有不同程度的凝集活性,其中对水生聚胞藻(Synechocystis aquetilis)的凝集活性最强,最低质量浓度只需0.0313g/L。Two lectins were purified from Porphyridiurn cruenturn and marine Chlorella by the method of ion exchange and gel filtration, and their agglutinations were conducted with cells from erythrocytes of animal and human blood group, species of marine and fresh microalgae, bacteria and fungi. The two lectins purified could agglutinate some cells or species of them in different degrees. Of these agglutinations, the strongest agglutinative activity at a minimum concentration of 0. 031 3 g/L was found toward Synechocystis aquetilis.

关 键 词：微藻 凝集素 细胞 凝集 

分 类 号：Q949[生物学—植物学]