小鼠胎肝基质细胞系MFLC自发分泌多种细胞因子  

MULTIPLE TYPES OF CYTOKINES SECRETED BY A MOUSE FETAL LIVER STROMAL CELL LINE

作　　者：刘倩[1] 刘祝公[1] 陈慰峰[1] 张平夏[1] 庞学文[1] 钱晓萍[1] 

机构地区：[1]北京医科大学免疫学系,北京100083

出　　处：《实验生物学报》1997年第4期355-361,共7页Acta Biologiae Experimentalis Sinica

摘　　要：在无外源刺激条件下,我室所建小鼠胎肝基质细胞系MFLC可自发分泌多种类型细胞因子,其中IL-6及化学趋化因子水平较高,GM-CSF水平较低,但未检测到IL-3及IL-7活性。此细胞上清对小鼠骨髓造血干细胞有明显的促集落形成效应,并呈现剂量依赖关系,所形成的集落以CFU-GMM及CFU-GM为主;此细胞上清还促进5-Fu耐受小鼠骨髓造血干细胞的集落形成,提示上清中存在SCF样活性成份。上述结果表明,MFLC的建立有利于分析干细胞在胎肝内如何向pro-T细胞分化发育的机理并有利于阐明细胞因子网络调节在其中的作用。A murine fetal liver stromal cell line, called MFLC. has been established and maintained in DME supplemented with 10%NCS for 2 years. Without exogenous stimulation, the MFLC cells spontaneously secreted several types of cytokines, including IL-6, GM-CSF and chemotactic factor. Of which, IL-6 and chemotactic factor were abundant, GM-CSF at low level. Biological activities of IL-7 and IL-3 were not detected in the MFLC cell's su-pernatants. MFLC-SN induced colony formation of murine bone marrow cells in a dose dependent fashion in which mixed granulocyte/macrophage/megakaryocy te co-lonies(CFU-GMM) and granulocyte/macro-phage colonies (CFU-GM) were dominant. MFLC-SN also sustained colony formation of bone marrow cells taken from 5-Fu injected mice, suggesting that there was a biological activity similar to SCF in MFLC-SN.The cytokines secreted by MFLC might play an important role in T cell early development in fetal liver. These results will be useful for us to analyse the mechanism of T cell development in fetal liver and to study the cytokine network regulation.

关 键 词：胎肝基质细胞 自发分泌 细胞因子集落 

分 类 号：R392.12[医药卫生—免疫学]