FK228促进IL-3介导的人红系前体细胞的增殖与分化  

FK228 promotes the IL-3 mediated proliferation and differentiation of human erythroid progenitor cells

作　　者：喻召才[1] 刘文超[1] 刘都户[1] 黄颖[1] 斯晓明[1] 尤向辉[1] 陈衍[1] 

机构地区：[1]第四军医大学西京医院肿瘤科,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2008年第7期672-675,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金青年科学基金资助项目(30500604)

摘　　要：目的:探讨组蛋白脱乙酰化酶抑制剂FK228在IL-3介导的人红系前体细胞增殖与分化中的调节作用。方法:经粒细胞集落刺激因子动员的肿瘤患者外周血单核细胞中分离CD34+细胞,用含干细胞生长因子(SCF)、白细胞介素3(IL-3)或SCF+IL-3及不同浓度FK228的无血清培养液培养7d,分别用抗CD14、GPA、CD15及CD36单克隆抗体(mAb)染色并行流式细胞术(FCM)检测;将CD34+细胞用含SCF、IL-3或SCF+IL-3及FK228的半固体培养液培养,并行细胞集落分析;将CD34+细胞用含SCF+IL-3的培养液培养7d,然后分离CD36+GPA-细胞,将细胞用含有IL-3+FK228的半固体培养液中培养,并行细胞集落分析。结果:FK228以一种剂量依赖方式促进CD36+细胞的产生,对CD14+细胞及CD15+细胞的产生无明显影响;0.5μg/LFK228可明显增加含IL-3培养体系中CD36+细胞的数量;FK228可明显增加含IL-3的半固体培养基中集落形成的数量及组成单个集落的细胞数;FK228可明显促进CD36+GPA-细胞在含IL-3的半固体培养基中形成红细胞集落。结论:FK228可促进白细胞介素3介导的人红系前体细胞的增殖与分化。AIM： To investigate the role of histone deacetylases （HDACs） inhibitor FK228 in the IL-3 mediated proliferation and differentiation of human erythroid progenitor ceils. METHODS： CD34＋ cells were separated from the granulocyte colony-stimulating factor（G-CSF） -mobilized peripheral blood of cancer patients and cultured for 7 days with FK228 of different concentrations, stem cell factor （SCF） andinterleukin 3 （IL-3） in serum-free medium. Flow cytometry was performed to analyze the expression of CD14, GPA, CD15 and CD36 on the cells and the colony assay of erythroid cells was conducted. Then the CD36 ＋ GPA- cells were endched and cultured with with IL-3 and 0.5 iJg/L FK228 supplemented serum-free semisolid cultures and the formation test of erythroid colony was conducted. RESULTS： FK228 enhanced the generation of CD36 ＋ cells in a dosedependent manner, in spite of that it displayed no influence on the generation of CD14＋ and CD15＋ cells. 0. 5 g/L FK228 induced a remarkable increase in the cell number of CD36＋ cells cultured with IL-3; FK228 also significantly increased the number and size of CD36＋ cells colony cultured with IL-3 containing semisolid cultures. Moreover, FK228 augmented the formation of CD36 ＋ cells colony from CD36 ＋ GPA- cells cultured with IL-3. CONCLUSION： FK228 can promote the IL-3 mediated proliferation and differentiation of human erythroid progenitor cells.

关 键 词：组蛋白脱乙酰化酶抑制剂 自细胞介素3 红系前体细胞 增殖 分化 

分 类 号：R392.11[医药卫生—免疫学]