黄体生成素类似物对离体大鼠颌下腺细胞分泌神经生长因子的影响  

Effects of LH analogs on the secretion of NGF in submaxillary gland cells of rats in vitro

作　　者：陈蕾[1] 白宏伟[2] 孙绪德[3] 赵军[1] 

机构地区：[1]海军总医院妇产科,北京100037 [2]海军总医院肝胆外科,北京100037 [3]第四军医大学唐都医院麻醉科,陕西西安710038

出　　处：《细胞与分子免疫学杂志》2008年第7期676-678,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：海军总医院院内基金资助(2007-2009)

摘　　要：目的:探讨黄体生成素(LH)类似物对离体大鼠颌下腺细胞分泌神经生长因子(NGF)的影响。方法:体外孵育大鼠颌下腺细胞并给予不同浓度的LH类似物,采用酶联免疫分析(ELISA)方法检测上清液中神经生长因子的含量。结果:当加入终浓度为10-6、10-4、10-2U/L的LH类似物孵育颌下腺细胞时,NGF的分泌量随着浓度的升高而逐渐增高;当LH类似物浓度为10-2、100、102U/L时,NGF的分泌量随LH类似物浓度的递减而降低;同时随着时间的增加,NGF的分泌量也会增加,孵育8h时达高峰,随后开始下降。结论:在体外LH类似物可双项调节大鼠颌下腺细胞分泌NGF的功能。AIM： To investigate the effects of luteinizing hormone （LH） analogs on the secretion of nerve growth factor （NGF） in submandibular gland cells of rats in vitro. METHODS： The submandibular gland cells of rats were incubated in vitro and LH analogs at various concentration were added to the medium. The never growth factor （NGF） was detected by enzyme-link immunoassay （ELISA）. RESULTS： When LH analogs were at the concentration of 10^-6, 10^-4, 10^-2 U/L respectively, the secretion of NGF was gradually increased. However, when LH analogs were at the concentration of 10^-2, 10^0, 10^2 U/L respectively, the secretion of NGF was gradually decreased. When LH analogs （10^-5 IU/mL） were incubated at different time, the secretion of NGF was different. NGF reached the peak value when it was incubated for 8 h and then decreased gradually. CONCLUSION： LH analogs may regulate the secretion of NGF in the submaxillary gland cells of rats.

关 键 词：LH受体 颌下腺 神经生长因子 ELISA 大鼠 

分 类 号：R962[医药卫生—药理学]