弓形虫多表位DNA疫苗的构建及其免疫保护作用  

Construction of multi-epitope DNA vaccine for Toxoplasma gondii and the study on protective immunity response in mice

作　　者：史霖[1] 刘珊[1] 程雁斌[1] 范桂香[1] 袁育康[1] 艾立[1] 

机构地区：[1]西安交通大学医学院免疫学及病原生物学系,陕西西安710061

出　　处：《细胞与分子免疫学杂志》2008年第7期689-691,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30571628)

摘　　要：目的:构建弓形虫多表位DNA疫苗并研究其免疫保护效果。方法:将编码含弓形虫多个T、B细胞表位的6段弓形虫多肽基因,以5个甘氨酸编码基因相间隔相连接,克隆入真核表达质粒pcDNA3.1(+)中,构建成多表位弓形虫DNA疫苗。免疫BALB/c小鼠,测定其诱导的特异性抗体水平及T淋巴细胞增殖状况,同时进行弓形虫攻击感染保护实验。结果:成功构建了包含多个弓形虫表位的真核表达质粒pcDNA3.1/T-ME,以其作为DNA疫苗免疫小鼠,可诱导机体产生弓形虫特异性的体液及细胞免疫应答,产生有效的抗弓形虫保护性免疫应答。结论:构建的弓形虫多表位DNA疫苗能诱导机体产生有效的保护性免疫应答,在控制弓形虫感染上具有可行性。AIM： To construct multi-epitope DNA vaccine for Toxoplasma gondii and study its protective immunity response. METHODS： The gene encoding six polypeptides of T. gondii, which consists of plenty of T and B epitopes, was cloned into the eucaryotic expression vector pcDNA3.1 （ ＋ ）. BALB/c mice were vaccinated by this multi-epitope based DNA vaccine （intramuscular needle injection）. The specific antibody and T cell proliferation were determined. Meanwhile, the DNA-vaccinated mice were challenged with a lethal dose of T. gondii tachyzoites for further observation. RESULTS： The eukaryotic expression plasmid （pcD- NA3.1/T-ME） encoding plenty of T, gondii epitopes was constructed successfully, pcDNA3.1/T-ME immunization induced T, gondii specific humoral and cellular immunity in mice. The mice immunized with pcDNA3.1/T-ME survived significantly longer than the mice in control after challenged by T, gondii RH strain infection. CONCLUSION. The multiepitope DNA vaccine can induce the protective immunity against T. gondii infection effectively in vivo, which is a potential strategy to control T, gondii infection.

关 键 词：弓形虫 表位 DNA疫苗 

分 类 号：R382.5[医药卫生—医学寄生虫学]