微卫星位点DYS19在中国人群中的多态研究  

A STUDY OF A POLYMORPHIC MICROSATELLITE LOCUS DYS19 IN CHINESE ETHNIC GROUPS

作　　者：包维东[1] 徐玖瑾[1] 朱苏玲[1] 许丽萍[1] 杜若甫[1] 

机构地区：[1]中国科学院遗传研究所

出　　处：《人类学学报》1997年第4期255-261,共7页Acta Anthropologica Sinica

基　　金：中国国家自然科学基金

摘　　要：以人ＤＮＡ为模板，经ＰＣＲ扩增后，用聚丙烯酰胺凝胶电泳分离扩增片段，再经高灵敏度银染着色，对中国陕西汉族、广东汉族、宁夏回族、辽宁满族、四川彝族、西藏藏族、广西壮族、广西瑶族、新疆维吾尔族、湖南土家族等１０个人群５３５名个体的Ｙ染色体上微卫星位点ＤＹＳ１９的遗传多态性进行了研究。结果表明：中国人群中以等位基因Ｃ（１９４ｂｐ）为主要等位基因，基因频率范围在０．２５—０．６１；等位基因Ｂ（１９０ｂｐ）、Ｄ（１９８ｂｐ）次之，基因频率范围分别为０．０８—０．３６、０．０６—０．４２；而等位基因Ａ（１８６ｂｐ）和Ｅ（２０２ｂｐ）频率较低，频率范围分别为０—０．０７和０—０．３８。在壮族中还检测出了一名携带Ｆ（２０６ｂｐ）等位基因的个体。中国人群ＤＹＳ１９等位基因的分布与蒙古人种群体以Ｃ型为主的结果相一致。χ２成对比较表明瑶族、藏族与大多数其它中国民族间ＤＹＳ１９表型分布存在差异（Ｐ＜０．０５）或显著性差异（Ｐ＜０．０１）。中国人群ＤＹＳ１９的基因频率至今在文献中尚未见报道。The distribution of a Y chromosome specific polymorphic loucs, DYS19, in 535 males of ten ethnic groups in China was studied by using PCR method followed by PAGE and silver staining The ten ethnic groups are Han in Shanxi Province, Han in Guangdong Province, Hui in Ningxia Hui Autonomous Region, Man in Liaoning Province, Uygur in Xinjiang Uygur Autonomous Region, Yi in Sichuan Province, Tibetan in Tibet Autonomous Region, Zhuang in Guangxi Zhuang Autonomous Region,Yao in Guangxi Zhuang Autonomous Region, Tujia in Hunan Province The result shows that allele C (194bp), frequency of which ranges from 0 25 to 0 61,is predominant in Chinese populations; alleles B (190bp) and D (198bp) take the second place with the frequency ranging from 0 08 to 0 36 and 0 06 to 0 42,respectively; while alleles A (186bp) and E (202bp) take the last place with the frequency ranging from 0 to 0 07 and 0 to 0 38, respectively An individual carrying the allele F (206bp) was identified in Zhuang The pairwise comparison with χ 2 test reveals that there exists difference (P<0 05) or significant difference (P<0 01) in DYS19 phenotype distribution between Yao、Tibetan and most other Chinese populations The allele frequency of DYS19 in Chinese ethnic groups has not been reported so far

关 键 词：中国民族 Y染色体 微卫星DNA DYS19 多态性 

分 类 号：Q987[生物学—遗传学]