实时可重构的共面型光诱导介电泳微操纵平台  

Real-time reconfigurable micro-manipulation platform using coplanar light-induced dielectrophoresis

作　　者：朱树存[1] 易红[1] 倪中华[1] 

机构地区：[1]东南大学机械工程学院,南京210096

出　　处：《仪器仪表学报》2008年第6期1143-1148,共6页Chinese Journal of Scientific Instrument

基　　金：国家863计划(2006AA04Z351);国家自然科学基金(50675033;30770553)资助项目

摘　　要：归纳与总结了各类单细胞微操作技术的最新研究进展,提出一种新的实时可重构的共面型光诱导操控平台,完成了光诱导介电泳芯片的设计、加工与操控平台的搭建。在通过采用机器视觉及模式识别技术实现微粒自动识别与实时追踪的基础上,通过视觉反馈控制在光电导层上生成动态可重构的光模式虚拟电极阵列,形成特定的空间势能形貌,并基于介电泳效应实现微粒的可编程操作。最后,基于多物理场耦合模型及数值模拟技术,定量分析了新式光诱导介电泳芯片的捕获及释放模式,并基于临界最大流速Q这一品质因数,对比分析了共面型与经典式光诱导介电泳捕获模型的尺寸选择性与槽道高度相关性。研究结果表明,共面型光诱导介电泳模型相对于经典光电子镊而言,在单细胞排布方面更具有优势——更高的捕获强度、稳定性、选择性以及分选效率。Following a review of the latest advancement in developing single-cell manipulation methods, a novel realtime reconfigurable manipulation platform using coplanar light-induced dielectrophoresis （LIDEP） was proposed. The design, microfabrication of the LIDEP chip and the experimental platform setup have been completep. The platform can automatically recognize and track microparticles using machine vision and pattern recognition. Close-loop control enables the programmable dielectrophoretic manipulation of microparticles through dielectrophoretic potentialenergy landscapes modulated by the dynamic reconfigurable virtual electrode patterns focused onto the photoconductive layer. Finally with the coupling model of multiphysics and the numerical simulation, a quantitative analysis was carried out for the trapping and releasing modes of coplanar LIDEP and with the quality factor of critical flow rate Q, the size-selectivity behavior and chamber height correlation for both coplanar LIDEP and optoelectronic tweezers （OET） were compared. The results show that the coplanar LIDEP chip holds advantage over OET in trap stiffness,trap stability and sorting efficiency.

关 键 词：光诱导介电泳 单细胞操控 多物理场耦合 

分 类 号：TP271[自动化与计算机技术—检测技术与自动化装置]