干旱胁迫对转BnDREB1-5基因烟草光合特性的影响  

The Effect on Photosynthetic Characteristics of Transgenic Tobacco with BnDREB1-5 Gene by Drought Stress

作　　者：刘卫群[1] 胡亚杰[1] 甄焕菊[2] 符云鹏[1] 刘巧真[3] 陈旭初 

机构地区：[1]河南农业大学国家烟草栽培生理生化研究重点实验室,河南郑州450002 [2]中国烟草总公司职工技术培训中心,河南郑州450008 [3]河南省农业科学院烟草研究中心,河南许昌461000 [4]河南省平顶山市烟草专卖局生产科,河南平顶山467002

出　　处：《武汉植物学研究》2008年第3期294-297,共4页Journal of Wuhan Botanical Research

基　　金：河南省科技攻关项目(0624050012)资助

摘　　要：检测了干旱胁迫对烟草(Nicotiana tabacum)叶片光合特性、叶绿素、类胡萝卜素和可溶性糖含量的影响。结果显示,干旱胁迫后转基因烟草的初始荧光(Fo)、非光化学猝灭系数(NPQ)增加幅度明显低于野生型烟草;其它荧光参数降低幅度明显小于野生型烟草;虽然干旱胁迫后两个烟草样品的光合参数、叶绿素含量都有所降低,但转基因烟草的降低幅度明显小于野生型烟草;干旱胁迫后,转基因烟草可溶性糖含量明显高于野生型烟草。表明BnDREB1-5转录因子具有抗干旱胁迫的作用。The photosynthetic characteristics,the content of chlorophyll, carotenoids and soluble sugar in tobacco leaves were analyzed after physical drought stressed. The results showed that after physical drought stressed,the initial fluorescence （Fo） and non-photochemical quenching （qN） of transgenic to-bacco increased, but were significantly lower than wild-type. Other fluorescence parameter lower rates were significantly lower than that of wild-type. The photosynthetic parameter and chorophyll content of both samples decreased, but the lower rates were significantly lower than that of wild-type. The content of soluble sugar of transgenic tobacco was significantly higher than wild-type. These results indicated that the BnDREB1-5 transcription factor gene had an effect on drought stressed.

关 键 词：烟草 BnDREB 干旱胁迫 光合特性 

分 类 号：Q78[生物学—分子生物学] S572[农业科学—烟草工业]