鸭病毒性肿头出血症病毒人工感染鸭的病理学观察  

Studies on the Pathomorphological Development of Experimental Duck Viral Swollen Head Hemorrhagic Disease

作　　者：李传峰[1] 程安春[1] 汪铭书[1] 沈婵娟[1] 张舍郁[1] 周毅[2] 陈孝跃[1] 

机构地区：[1]四川农业大学动物医学院禽病防治研究中心,雅安625014 [2]动物疫病与人类健康四川省重点实验室,雅安625014

出　　处：《畜牧兽医学报》2008年第6期764-770,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技攻关重大项目(2004BA901A03);教育部“新世纪优秀人才支持计划”项目(NCET-04-0906/NCET-06-0818);四川省重大基础研究项目(04JY029-006-1/07ZQ026-132);四川省重点建设学科项目(SZD0418)

摘　　要：72只28日龄樱桃谷鸭肌注感染鸭病毒性肿头出血症病毒（DSHDV）,于不同阶段解剖感染鸭和发病死亡鸭,应用光镜和电镜观察各器官的组织学和超微结构变化。结果：①光镜：感染前期（2-72 h）各组织充血、出血,并伴有数量不等的炎性细胞浸润;后期（119-170 h）免疫器官（脾脏、胸腺、法氏囊、哈德氏腺）、消化器官（肝、胰）、肾、大脑组织细胞坏死或出现大小不等的坏死灶;消化道（食管、腺胃和各段肠管）和气管黏膜上皮细胞完全坏死脱落,固有膜严重充血、出血;心脏肌纤维间弥漫性出血,肺脏和胸肌组织小血管充血。②电镜：主要表现为细胞核空化,胞质内各种细胞器肿胀、扩张,甚至溶解、消失,有的形成含有较多病毒粒子的＂封入体＂。研究表明法氏囊、胸腺、脾脏、肝脏、消化道和气管为DSHDV感染的主要靶器官。肝细胞、淋巴细胞、成纤维细胞、胆管上皮细胞、消化道上皮细胞为DSHDV侵害的靶细胞。72 Ying-Tao-Gu ducks of 28-day-old were infected with duck viral swollen head hemorrhagic disease virus （DSHDV） by muscle injection. The sick ducks and the dead ducks were dissected at different time to study the changes of histology and ultrastructure of tissues by using light microscopic techniques and electron microscopic techniques. The results of light microscopic observation： At the early stage of infection （2-72 h）, the congestion, hemorrhage and the infiltration of inflammatory ceils in different number were found in tissues. At the last course of the disease （119-170 h）, necrotic lesions of different sizes were seen in immune organs （spleen, thymus, bursa of Fabricius and gland of Harder）, digestive organs （liver and pancreas）, kidney and cerebrum tissues, digestive tract （oesophagus, stomachus glandularis and all intestinal canals） and windpipes epithelium mucosaes completely suffered with necrosis and desquamation, lamina propria showed severe congestion and hemorrhage; Diffuse hemorrhage of heart as well as engorgement of the small vessels of lung and pectoralis were seen clearly. The results of electron microscope observation： The main ultrastructural changes were the cavitation of cell nucleus, swelling and expanding of all kinds of organelles, even some dissolved and disappeared. The Occlusionbody was formed in cytoplasm and a large amount of virions filled in it. The results maked it clear that bursa of Fabricius, thymus, spleen, liver, tractus atimentarius and windpipes were the target organs that DSHDV attacked mainly. The main target cells that DSHDV attacked were hepatocyte, lymphocyte, collagenoblast, bile duct endothelial cell and digestive tube endothelial cells.

关 键 词：鸭病毒性肿头出血症病毒 人工感染 鸭 组织学 

分 类 号：S852.659.4[农业科学—基础兽医学]