四君子汤发酵液的抗肿瘤作用及其对荷瘤小鼠免疫功能的影响  

Immunomodulation and Antitumor Activity of Fermented Decoction of Sijunzi in S180-bearing Mice

作　　者：韩春杨[1] 林德贵[1] 刘翠艳[2] 郭顺星[3] 

机构地区：[1]中国农业大学动物医学院,北京100193 [2]山东农业大学动物科技学院,泰安271018 [3]中国医学科学院中国协和医科大学药用植物研究所,北京100193

出　　处：《畜牧兽医学报》2008年第6期814-818,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然基金项目(30471275)

摘　　要：本试验研究了四君子汤发酵液对S180肿瘤模型小鼠的抗肿瘤作用和对免疫功能的影响。采用血清溶血素法检测荷瘤小鼠体液免疫功能;MTT比色法检测淋巴细胞的增殖和流式细胞术检测T细胞亚群。结果表明,四君子汤发酵液具有明显的抑制肿瘤生长的作用,并且可以增强环磷酰胺(CTX)的抗肿瘤作用,降低CTX免疫抑制的副作用。四君子汤发酵液可以提高免疫器官指数;对肿瘤小鼠脾淋巴细胞转化、血清抗体产生都有促进作用;可以明显提高模型小鼠CD3+和CD8+T淋巴细胞水平,降低由于肿瘤引起的CD4+/CD8+T淋巴细胞比值的升高,与模型组比较差异显著(P<0.05)。研究表明四君子汤发酵液的抗肿瘤作用和增强机体免疫调节功能有关。The immunomodulation and antitumor activity of fermented decoction of Sijunzi （FDSJZ） in S180-bearing mice was investigated. The mice inoculated with S180 cell suspension were treated with FDSJZ for 10 days. The homolysin assays, MTT colorimetric method and flow cytometry were used to study the humoral immunity and cellular immunity of S180-bearing mice. The results showed that FDSJZ could significantly inhibit the growth of transplantable sarcoma S180 and it could enhance the anti-tumor activity of CTX, and lower the side effect of CTX. FDSJZ could increase organ indices, spleen lymphocyte proliferation, the form of antibody secreted by spleen cells, and the percent of CD3^＋ and CD8^＋ of S180-bearing mice. And it could lower the ratio of CD4^＋/CD8^＋ which was elevated abnormally in S180-bearing mice. The results showed that there was some relation between the antitumor activity of FDSJZ and the immunomodulation.

关 键 词：四君子汤发酵液 S180 抗肿瘤 免疫调节功能 

分 类 号：S853.73[农业科学—临床兽医学]