鸭A-FABP基因的克隆及其组织表达  

Cloning and Tissue Specific Expression of Duck A-FABP Gene

作　　者：张军[1] 董飚 张红[1] 储冬生[1] 徐国庆[1] 龚道清[1] 段修军 赵旭庭 顾志良[3] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]国家级水禽种质资源基因库,泰州225300 [3]常熟理工学院生物系,常熟215500

出　　处：《畜牧兽医学报》2008年第6期819-822,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：江苏省自然科学基金资助项目(BK2004055);江苏省高校自然科学基金资助项目(06KJB230130)

摘　　要：本文采用RT-PCR方法从35周龄母鸭脂肪组织中克隆了A-FABP基因的cDNA序列,其长度为422 bp,包含了1个399 bp的完整CDS,编码132个氨基酸。该序列与家禽（鹅、鸡）和哺乳动物（人、小鼠、猪、牛）的A-FABP基因序列约有94%和73.4%-75.7%的同源性,而相应氨基酸序列的同源性为92.4%和72.0%-77.3%。半定量RT-PCR研究结果为A-FABP基因在间脑、肺和肾组织中不表达,在脂肪组织中的表达明显高于其他组织（P〈0.5）。表明A-FABP基因在动物进化中具有高度保守性,该基因的表达具有组织特异性。A 422 bp eDNA of A-FABP gene was cloned and sequenced from the adipose tissue of a 35-week-old female duck, it contains a complete 399 bp CDS, encoding 132 amino acids. The duck A-FABP gene shared about 94% homology with that of poultry（goose and chicken）, and 73.4%-75.7% homology with that of mammal （human, mouse,pig and cattle）, while the corresponding protein shared more than 92.4% and 72.0%-77.3% homology with the above animals＇ A-FABP protein, respectively. The results of the semi-quantity RT-PCR indicated that duck AFABP mRNA did not expressed in diencephalons, lung and kidney, and its mRNA expression level of adipose tissue was higher than that of other tissues （P〈0.5）. A-FABP gene is of high conservation in the course of animal evolution. The expression level of A-FABP mRNA is particular to tissues.

关 键 词：鸭 A-FABP基因 克隆 组织表达 

分 类 号：S834.2[农业科学—畜牧学]