绞股蓝总皂苷对谷氨酸所致大鼠海马组织损伤的保护作用  

Protective effects of gypenosides against glutamate-induced hippocampus injury in rats

作　　者：韩玉霞[1] 魏欣冰[1] 李霞[2] 丁燕[1] 辛华[2] 丁华[1] 

机构地区：[1]山东大学医学院药理学研究所,济南250012 [2]山东大学医学院细胞生物学研究所,济南250012

出　　处：《山东大学学报（医学版）》2008年第5期449-452,共4页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助课题(Y2006C41)

摘　　要：目的探讨绞股蓝总皂苷(gypenosides,GP)对大鼠侧脑室注射谷氨酸(glutamate,Glu)引起的海马组织氧化损伤的保护作用。方法将大鼠随机分为假手术组、Glu损伤组、GP低(200 mg/kg)、中(400 mg/kg)、高(800 mg/kg)剂量组。GP组预先灌胃给药10 d。大鼠经侧脑室注射Glu后2 h取脑,测定各组大鼠海马组织匀浆中的丙二醛(MDA)含量、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及产生羟自由基的能力;制备海马组织切片,观察各组形态学变化。结果Glu损伤组大鼠海马组织中MDA含量和羟自由基生成能力均高于假手术组(P<0.05),而SOD和GSH-Px活性降低(P<0.05);与Glu损伤组比较,GP组MDA含量和产生羟自由基的能力降低(P<0.01),SOD和GSH-PX活性明显增强(P<0.01);HE染色显示,GP组海马神经元变性及坏死较Glu损伤组明显减少。GP 400 mg/kg对Glu所致海马组织氧化损伤的保护作用最为明显。结论GP通过增强抗氧化酶活性,抑制羟自由基和脂质过氧化物生成而减轻Glu介导的氧化性神经损伤,发挥神经保护作用。Objective To investigate the protective effects of gypenosides（GP） against glutamate-induced oxidative injury to the hippocampus of rats. Methods 60 Wistar rats were randomly divided into 5 groups： the sham-operation group, the glutamateinduced injury group, the GP low （200 mg/kg）, medium（400 mg/kg） and high （800 mg/kg） dose groups. Intra-gastric administration of GP was used for 10 d. 2 h after injection with glutamate through the lateral ventricle, the homogenate of the hippocampus was collected and the MDA, SOD, GSH-Px activity and the hydroxyl radical generation capacity were determined. Results： The MDA concentration and hydroxyl radical generation capacity were significantly increased（ P 〈 0.05）, however activities of SOD and GSH-Px were significantly decreased （ P 〈 0.01）in the glutamate-induced injury group compared with the sham-operation group. Also, the MDA concentration and hydroxyl radical generation capacity were significantly decreased in the GP groups compared with the glutamate-induced injury group （ P 〈 0.01 ）, and activities of SOD and GSH-Px were significantly increased （P 〈 0.01） by GP treatments, and 400 mg/kg GP had the most effective action. Conclusion The protective effects of GP against Glu-mediated nerve injury may be mediated by increasing the activity of antioxidant enzymes and inhibiting the hydroxyl radical production.

关 键 词：绞股蓝总皂苷 谷氨酸 氧化性神经毒性 

分 类 号：R965[医药卫生—药理学]