眼眶及眼附属器黏膜相关B细胞淋巴瘤中BCL10表达和染色体易位的检测  

Abnormal BCL10 nuclear expression and chromosomal translocation in ocular adnexal mucosaassociated lymphoid tissue lymphomas

作　　者：李百周[1] 杨文涛[1] 周晓燕[1] 范月珍[1] 陆洪芬[1] 施达仁[1] 

机构地区：[1]上海复旦大学附属肿瘤医院病理科,200032

出　　处：《中华眼科杂志》2008年第6期545-548,共4页Chinese Journal of Ophthalmology

摘　　要：目的研究眼眶及眼附属器黏膜相关（OA—MALT）淋巴瘤中BCL10蛋白表达及染色体易位情况。方法收集60例OA—MALT淋巴瘤患者的标本，采用免疫组织化学法检测BCL10蛋白的表达，用荧光素原位杂交（FISH）法分别检测AP12-MALT1融合基因、BCL10、MALT1及1gH基因的异常。采用交叉分组设计，对结果进行Fisher精确检验。结果OA—MALT淋巴瘤中BCL10蛋白表达的阳性率为85．0％（51／60），其中胞质阳性率为41．7％（25／60），胞核阳性率为43．3％（26／60）。FISH结果显示除2例患者有AP12-MALT1融合基因外，其余患者的BCL10基因和IgH基因均未发现异常。统计学分析显示OA—MALT淋巴瘤中BCL10蛋白核表达不依赖于染色体异常（P=0．184）。结论OA—MALT淋巴瘤中有较高的BCL10蛋白核表达率，对OA—MALT淋巴瘤的诊断有一定的辅助价值。在全身其他部位MALT淋巴瘤中报道的染色体易位，在OA—MALT淋巴瘤中并不常见。Objective To detect the BCL10 expression and several types of chromosomal translocations [ including t （ 11 ; 18 ）/API2-MALT1 ; t （ 1 ; 14）/IgH- BCL10 and t （ 14 ; 18 ）/MALT1 -IgH ] in ocular adnexal mucosa-associated lymphoid tissue lymphomas （OA-MALT lymphomas）. Methods Sixty OA-MALT lymphomas were collected from Cancer Hospital of Fudan University. BCL10 was detected by immunohistochemical studies, and API2-MALT1 fusion gene, BCL10, MALT1 and IgH chromosomal abnormalities were detected by fluorescent in situ hybridization （ FISH ）. Fisher＇s exact test was used to analyze the relation between nuclear BCL10 expression and chromosomal translocation. Results BCL10 expressed in 85. 0% （51/60） OA-MALT lymphomas. Among these positive cases, 25 cases （41.7%） were expressed in the cytoplasm, and 26 cases （43.3%） were expressed in the nucleus. FISH results showed that no chromosomal abnormalities related with BCL10 and IgH genes, except 2 cases with API2-MALT1 fusion gene. Under statistic of Fisher exact test, nuclear BCL10 expression and API2-MALT1 fusion gene were two independent factors （ P 〉 0. 05 ）. Conclusions BCL10 nuclear expression is common in OA- MALT lymphomas and may be used as a potential marker for the diagnosis of MALT lymphomas arising from ocular adnexa. Aberrant chromosomal translocations reported in the other sites MALT lymphomas are rare in OA-MALT lymphomas.

关 键 词：淋巴瘤 黏膜相关淋巴样组织 眼肿瘤 衔接蛋白质类 信号传导 易位 遗传 免疫组织化学 原位杂交 荧光 

分 类 号：R686[医药卫生—骨科学]