早期心肌缺血猝死心肌NF-κB p65的表达研究  

The study on the nuclear factor-κB p65 expression of sudden death in the early stage of acute myocardial ischemic

作　　者：高淑红[1] 刘聪[2] 谭秋丰[1] 黄飞骏[1] 

机构地区：[1]四川大学华西基础医学与法医学院,四川成都610041 [2]四川大学华西医院感染性疾病中心,四川成都610041

出　　处：《中国法医学杂志》2008年第3期145-147,I0001,共4页Chinese Journal of Forensic Medicine

摘　　要：目的探讨核因子NF-κBp65(NF-κB p65)在心肌早期缺血猝死后诊断中的法医学价值。方法将收集的案例心肌蜡块分为3组:正常对照组(3例)、早期心肌缺血组(14例)、心肌梗死组(8例),采用免疫组织化学技术(SP法),观察猝死心肌内NF-κBp65的表达情况,并对其结果进行半定量分析。结果早期心肌缺血组和心肌梗死组的心肌细胞胞浆内及细胞核内均出现NF-κBp65的表达,且早期心肌缺血组与心肌梗死组表达强度无差异。结论NF-κBp65可以作为早期心肌缺血猝死的一个辅助诊断指标。Objective To investigate the forensic applicability of nuclear factor-kappa B p65 （NF-κB p65） to the diagnosis of early myocardial ischemia in cases of sudden cardiac death. Methods The cases were divided into three groups： the cases with early myocardial ischemia, myocardial infarction and cases used as control. Immunostaining of NF-κB p65 was performed by SP method in cardiac tissues. The intensity of NF-κB p65 was observed under light microscope and analyzed semi-5quantitatively. Results NF-κB p65-positive cardiac cells were detected in cytoplasm and nuclei in the cases with early myocardial ischemia and myocardial infarction, and the intensity of immunoreactivity was approximately the same. No positive immunostaining was found in the control cardiac tissues. Conclusion The results suggest that the early myocardial ischemia may have revealed as the same changes as myocardial infarction at molecular level, indicating that NF-κB p65 may be used as a marker for postmortem diagnosis of the early acute myocardial ischemia by immunohistochemical technique.

关 键 词：法医病理学 心脏性猝死 NF-κB P65 免疫组织化学 

分 类 号：D919[医药卫生—法医学]