缝隙连接蛋白43与创伤后脑水肿相关性研究  

Study on correlation between connexin 43 and brain edema in experimental brain injury

作　　者：吴中亮[1] 廖春华[1] 任宁[2] 

机构地区：[1]第四军医大学西京医院神经内科,陕西西安710032 [2]第四军医大学西京医院综合诊疗科,陕西西安710032

出　　处：《中华神经外科疾病研究杂志》2008年第3期201-204,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：全军"十一五"医药卫生科研基金资助项目(06MA227)

摘　　要：目的探讨缝隙连接蛋白43(Cx43)与创伤后脑水肿的相关性。方法大鼠脑损伤前2 h经侧脑室注射Cx43特异性阻断剂反义寡核苷酸(ODNs)或生理盐水,用Feeney's自由落体法制作局灶性脑损伤动物模型,通过星形胶质细胞的特异性标记物-胶质纤维酸性蛋白(GFAP)免疫荧光染色,观察脑皮质区星形胶质细胞的形态、数量的变化,同时观察脑组织的水肿情况。结果脑损伤后生理盐水对照组GFAP标记的星形胶质细胞数量较ODNs预处理组明显增多(P<0.05),细胞胞体较肥大;ODNs预处理组脑水肿较生理盐水对照组明显减轻(P<0.05)。结论阻断Cx43可减轻脑创伤后脑水肿的程度,Cx43参与了脑损伤后的脑水肿的形成。Objective To explore the correlation between connexin 43 （Cx43） and brain edema in the experimental brain injury. Methods After injecting antisense oligodeoxynucleotides （ODNs, Cx43 blocker） or isotonic Na chloride into lateral ventricle, rat focal brain injured models were made using Freeney＇s method. Single anti-glial fibrillary acidic protein （GFAP） immunofluorescent method was used to observe the expression of GFAP in cerebral cortex after brain injury. Results The GFAP positive cells in the saline-treated control group increased significantly compared with those in the rats pretreated with ODNs （P 〈 0. 05 ）. Brain edema in the saline-treated control group was mere obvious than that of the pretreated with ODNs group （ P 〈 0. 05 ）. Conclusion Cx43 can exacerbate brain edema after brain injury and participate in the secondary brain injury.

关 键 词：脑损伤 脑水肿 星形胶质细胞 缝隙连接蛋白43 

分 类 号：R651.15[医药卫生—外科学]