α-1,3-半乳糖苷转移酶基因沉默小鼠的建立初探  

Production of α-1,3-GT Transgenic Mice Through Microinjection

作　　者：李莉[1] 刘西梅[1] 谷习文[1] 肖红卫[1] 王亚刚[1] 田永祥[1] 郑新民[1] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所,湖北省动物胚胎工程及分子育种重点实验室,武汉430064

出　　处：《实验动物与比较医学》2008年第3期144-147,共4页Laboratory Animal and Comparative Medicine

基　　金：国家自然科学基金项目(No.30671495)

摘　　要：目的获得转α-1,3-半乳糖苷转移酶沉默基因的小鼠。方法将α-1,3-半乳糖苷转移酶沉默基因用显微注射的方法导入小鼠受精卵。结果移植注射胚853枚,其中移植312枚1-细胞胚于11只受体鼠,6只受孕(54.5%)均中途流产(解剖发现子宫角明显增粗、充血、残骸);移植注射胚541枚2-细胞胚于23只受体鼠,18只受孕(78.3%),11只受孕均中途流产了,3只足日剖宫产获14只死胎,平均体重2.14g,未检测到阳性;4只12日龄左右剖宫获21只残骸有7只阳性。结论通过显微注射的方法,α-1,3-半乳糖苷转移酶沉默基因可以整合,但要得到整合成活个体还有待进一步研究。Objective To product α-1,3-GT transgenic mice animal model for the study ofxenograft. Method The α- 1,3-GT genes was introduced into fertilized mice eggs of one-cell stage by microinjection. Result Eight hundred and fifty-eight fertilized eggs were introduced with α-1,3-GT gene. Of them, 312 fertilized mice eggs of one-cell stage were transplanted into 11 pseudpregnant mice, 6 of them were pregnanted （54.5%） and were aborted; 541 fertilized mice eggs of two-cell stage were transplanted into 23 pseudpregnant mice, 18 of them were pregnanted （78.3%） and 11 pregnant females were aborted, 14 dead offspring were born from three pregnant females. None were proved to be gene integration positively; 21 dead offspring were born from four pregnant females of days or so, 7 of them were proved to be gene integration positively. Conclusion Transgenic mice have been obtained through microinjection of α-1, 3-GT genes, and further study needed to product the live α-1,3-GT transgenic mice offspring .

关 键 词：α-1 3-半乳糖苷转移酶(α-GT) 基因沉默 小鼠 小干扰RNA 转基因 

分 类 号：Q78[生物学—分子生物学]