西藏小型猪MSTN基因5'调控区的酶切多态性分析  

The Study of Tibet Mini-pigs Myostatin Gene in 5' Controllable Region by PCR-RFLP

作　　者：岳敏[1] 顾冬生[2] 李洪涛[1] 张建明[1] 李金泽[1] 王玉珏[1] 顾为望[1] 

机构地区：[1]南方医科大学实验动物中心暨比较医学研究所,广州510515 [2]中国人民解放军421医院,广州510318

出　　处：《实验动物与比较医学》2008年第3期155-159,共5页Laboratory Animal and Comparative Medicine

基　　金：广东省农业攻关计划(2006B20201053);广东省科研条件建设项目(2006B60101059)

摘　　要：目的对西藏小型猪MSTN基因进行PCR-RFLP基因多态性分析,并对五指山小型猪、贵州香猪和巴马小型猪进行MSTN基因多态性的比较研究。方法以42头西藏小型猪、17头五指山小型猪、7头巴马小型猪和22头贵州香猪作为研究对象,对猪MSTN基因5′调控区进行了PCR-RFLP多态性研究。结果1、不同猪种之间的基因型频率和等位基因频率有显著性差异(P=0.000),西藏小型猪的MSTN基因表现出3种基因型TT(9.52%)、AA(61.90%)、TA(28.57%),T和A的频率分别为23.81%和76.19%;五指山小型猪的MSTN基因表现出2种基因型TT(82.35%)、AA(17.65%),T和A的频率分别为82.35%和17.65%;贵州香猪的MSTN基因表现出2种基因型TT(81.82%)、TA(18.18%),T和A的频率分别为90.91%和9.09%;巴马小型猪的MSTN基因表现出2种基因型TT(71.43%)和TA(28.57%),T和A的频率为85.71%和14.29%。2、不同MSTN基因型的西藏小型猪的初生重(P=0.761)和离乳重(P=0.319)无显著性差异。结论西藏小型猪、五指山小型猪、贵州香猪和巴马小型猪的MSTN基因5'调控区存在多态性,但暂不能把MSTN基因做为西藏小型猪繁殖性能的分子标记。Objectives To analyze the MSTN gene of Tibet mini-pigs, WU Zhi-shan mini-pigs, Bama mini-pigs and Guizhou mini-pigs. Methods Taking 42 Tibet mini-pigs, 17 Wu Zhi-shan mini-pigs, 7 Bama mini-pigs and 22 Guizhou mini-pigs as samples, PCR-RFLP polymorphism analysis on the controllable region of the MSTN gene 5＇ of pigs were carried out. 42 Tibet mini-pigs were used to analyze the relation between the RFLP polymorphism of MSTN gene and some breeding characters （weight at birth （WB） and weaning weight（WW））. Results （1）There is significant difference in genetype frequencies and allele frequencies between different stocks of pigs （P=0.000）. Tibet mini-pigs have three MSTN genetypes TT（9.52%）, AA（61.90%）, TA（28.57%）, and the frequencies of alleles T and A is 23.81% and 76.19% respectively; Wu Zhi-shan mini-pigs have two genetypes TT（82.35%）, AA （17.65%）, and the frequencies of alleles T and A is 82.35% and 17.65% respectively; Guizhou minipigs have two genetypes TT（81.82%）, TA（18.18%）, and the frequencies of alleles T and A is 90.91% and 9.09% respectively; Bama mini-pigs have two genetypes TT（71.43%）, TA（28.57%）, and the frequencies of alleles T and A is 85.71% and 14.29% respectively. （2） The difference of WB （P=0.761） and WW（P=0.319） is not significant between Tibet mini-pigs with different MSTN genetype. Conclusions The polymorphism are found in controllable region ofmyostatin gene 5＇ of Tibet mini- pigs, WU Zhi-shan mini-pigs, Bama mini-pigs and Guizhou mini-pigs. But MSTN gene can＇t be regarded as molecular mark for breeding character of the Tibet miniature pigs at present.

关 键 词：西藏小型猪 五指山小型猪 巴马小型猪 贵州香猪 MSTN基因 PCR-RFLP 

分 类 号：Q953[生物学—动物学]