白背三七叶中吡咯里西啶生物碱的LC-MS^n检测  

Detection of pyrrolizidine alkaloids from leaf of Gynura divaricata by LC-MS^n

作　　者：李丽梅[1] 张涵庆[1] 吕寒[1] 李维林[1] 徐德然[2] 郭巧生[3] 

机构地区：[1]江苏省·中国科学院植物研究所(南京中山植物园),江苏南京210014 [2]中国药科大学中药学院,江苏南京210038 [3]南京农业大学中药材研究所,江苏南京210095

出　　处：《植物资源与环境学报》2008年第2期79-80,共2页Journal of Plant Resources and Environment

基　　金：国家自然科学基金资助项目(30470190);南京市科学技术局现代农业项目(200501015)

摘　　要：The pyrrolizidine alkaloids(PAs) in leaf of Gynura divaricata(L.) DC.was analyzed by LC-MSn technique.The result shows that two hepatotoxic pyrrolizidine alkaloids(HPAs) are detected from the methanol extract of G.divaricata leaf,with relative molecular weight of 335 and 365,respectively and lower content.The constituent with relative molecular weight 335 is a retronecine-type alkaloid,and identified as integerrimine;another constituent with relative molecular weight 365 is an otonecine-type alkaloid,speculated to be isomer of usaramine or 18-hydroxyinterrimine.The pyrrolizidine alkaloids （PAs） in leaf of Gynura divaricata （L.） DC. was analyzed by LC-MSn technique. The result shows that two hepatotoxic pyrrolizidine alkaloids （HPAs） are detected from the methanol extract of G. divaricata leaf, with relative molecular weight of 335 and 365, respectively and lower content. The constituent with relative molecular weight 335 is a retronecine-type alkaloid, and identified as integerrimine; another constituent with relative molecular weight 365 is an otonecine-type alkaloid, speculated to be isomer of usaramine or 18-hydroxyinterrimine.

关 键 词：白背三七 吡咯里西啶生物碱 全缘千里光碱 液相色谱与多级质谱联用法(LC—MS^n) 

分 类 号：Q946.88[生物学—植物学] R284.2[医药卫生—中药学]