肺纤方颗粒对盐酸平阳霉素致肺间质纤维化大鼠血清转化生长因子β1和血小板衍生生长因子的影响  

Influence of Feixian Formula on serum transforming growth factor-β1 and platelet-derived growth factor in rats with bleomycin-induced pulmonary fibrosis

作　　者：张伟[1] 姜良铎[1] 张晓梅[2] 吴建军 邓媛[4] 鲁香凤[5] 

机构地区：[1]北京中医药大学东直门医院呼吸热病科,北京100700 [2]北京中医药大学东方医院呼吸热病科,北京100078 [3]北京王府中西医结合医院中医综合科,北京102209 [4]中国中医科学院中医杂志社,北京100700 [5]北京中医药大学东直门医院病理科,北京100700

出　　处：《中西医结合学报》2008年第6期595-599,共5页Journal of Chinese Integrative Medicine

基　　金：北京市科学技术委员会课题(No.Y0204003041011)

摘　　要：目的:观察肺纤方颗粒抗大鼠肺间质纤维化的作用,及其对大鼠血清转化生长因子β1(transforming growth factor-β1,TGF-β1)和血小板衍生生长因子(platelet-derived growth factor,PDGF)的影响。方法:72只雄性Wistar大鼠经气管插管灌注盐酸平阳霉素1mg/kg复制大鼠肺间质纤维化模型,随机分入模型组、泼尼松组和肺纤方组,每组24只;15只雄性Wistar大鼠经气管插管灌注等体积生理盐水作为假手术组。造模后24h开始常规灌胃给药,泼尼松组给予醋酸泼尼松5mg/kg,肺纤方组给予肺纤方颗粒1.25g/kg,模型组和假手术组给予等体积生理盐水,1次/d。分别于第14、28、45天取材,假手术组每批5只,其余各组每批8只。检测其肺组织病理改变及血清中TGF-β1和PDGF的水平。结果:与模型组和泼尼松组比较,在第45天时病理检测显示肺纤方组肺间质纤维化的程度较轻;血清TGF-β1和PDGF检测结果显示,模型组大鼠两种因子水平升高,第45天时升高最明显,并高于假手术组(P<0.05)。肺纤方组与泼尼松组的TGF-β1变化与模型组相似(P>0.05),两组间TGF-β1变化比较差异无统计学意义(P>0.05)。肺纤方组PDGF水平在第14天时与其余各组比较显著升高(P<0.01),然后持续下降,至第45天与假手术组接近(P=0.792);而模型组和泼尼松组PDGF水平皆随时间延长逐渐上升,第45天时均显著高于假手术组(P<0.01)。结论:肺纤方有一定的减轻盐酸平阳霉素致大鼠肺间质纤维化的作用,其作用机制可能与对血清PDGF水平的调节作用有关。Objective： To observe the effects of Feixian Formula, a compound traditional Chinese herbal medicine for treating pulmonary fibrosis, on bleomycin-induced pulmonary fibrosis in rats, and its influence on serum transforming growth factor-β1 （TGF-β1） and platelet-derived growth factor （PDGF）. Methods： Seventy-two male Wistar rats were infused with bleomycin （1 mg/kg） through tracheal intubation to induce pulmonary fibrosis, and they were randomly divided into untreated group （n =24）, prednisone-treated group （n=24） and Feixian Formula-treated group （n=24）. Fifteen male Wistar rats of the sham-operated group were infused with equivalent normal saline. Twenty-four hours after operation, prednisone （5 mg/kg） and Feixian Formula （1, 25 g/kg） were given to the prednisone-treated group and Feixian Formula-treated group respectively by intragastric administration once a day. Equivalent saline was administered to rats of the untreated group and sham-operated group. On the 14th, 28th and 45th day, 5 rats in the sham-operated group and 8 rats in each of the other three groups were dissected to observe pathologic changes of the lung tissues, and the levels of serum TGF-β1 and PDGF were determined by enzyme-linked immunosorbent assay. Results： At the 45th day, the degree of pulmonary interstitial fibrosis was lesser in rats of the Feixian Formula- treated group as compared with those of the untreated group and prednisone-treated group. The levels of serum TGF-131 and PDGF were increased, and were significantly higher than those of the sham-operated group, especially on the 45th day （P〈0.05）. Changes of TGF-β1 level in the prednisone-treated group and the Feixian Formula-treated group were similar to untreated group （P〉0.05）, and there was no significant difference between the prednisone-treated group and the Feixian Formula-treated group （P〉0.05）. PDGF in the Feixian Formula-treated group reached the highest level on the 14th day, significantly higher than those of th

关 键 词：肺纤方颗粒 肺疾病 间质性 肺纤维化 转化生长因子Β1 血小板衍生生长因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]