巢式PCR在鉴别多房棘球绦虫及细粒棘球绦虫基因亚型中的应用  

Application of Nest PCR in Differentiating Genotype of Echinococcus granulosus and Echinococcus multilocularis

作　　者：张亚楼[1] 刘开泰[2] 刘继文[3] 杨晓燕[3] 温浩[1] 苗玉清[1] 马旭东[1] 齐新伟[1] 刘涛[1] 

机构地区：[1]新疆医科大学一附院医学研究中心,乌鲁木齐830054 [2]中国疾病预防控制中心教育培训处,北京100050 [3]新疆医科大学公共卫生学院,乌鲁木齐830054

出　　处：《地方病通报》2008年第3期1-2,7,共3页Endemic Diseases Bulletin

基　　金：国家自然科学基金(30760228);乌鲁木齐市科学技术计划项目(y06231003);新疆医科大学第一附属医院科研奖励基金(2007-YFY-04)

摘　　要：目的探索巢式PCR在鉴别多房棘球绦虫及细粒棘球绦虫基因亚型中的应用价值。方法将水泡带绦虫、犬弓首蛔虫、多房棘球绦虫、细粒棘球绦虫羊株和骆驼株等样本的DNA用巢式PCR扩增。结果巢式PCR可以扩增出多房棘球绦虫和水泡带绦虫,而对细粒棘球绦虫羊株和骆驼株及其他寄生虫均不能扩增出。结论在鉴别细粒棘球绦虫和多房棘球绦虫方面,巢式PCR有一定的诊断意义,但不能用于鉴别细粒棘球绦虫基因亚型。Objective To explore the role of the nest polymerase chain reaction （ PCR ） in differentiating genotype of Echinococcus granulosus and Echinococcus multilocularis. Methods Samples of Echinococcus granulosus sheep strain, Toxocara canis, Taenia hydatigena, Echinococcus granulosus/multilocularis mixed sample, Echinococcus sheep strain/camel strain mixed samples were used for experiment. Results The nest PCR could amplified Echinococcus multilocularis and Taenia hydatigena＇s DNA samples. Echinococcus sheep strain and/or camel strain and others parasites＇DNA samples could not be amplified by the nest PCR. Conclusions The results show that the nest PCR may play a role in differentiation of Echinococcus granulosus and Echinococcus multilocularis. And the method could not be used in differentiation of Echinococcus granulosus genotype.

关 键 词：细粒棘球绦虫 多房棘球绦虫 特异性 基因分型 

分 类 号：R383.33[医药卫生—医学寄生虫学] Q75[医药卫生—基础医学]