长春新碱诱发外周神经病理性疼痛模型的建立  

STUDY ON THE RAT MODEL OF VINCRISTINE-INDUCED PERIPHERAL NEUROPATHIC PAIN

作　　者：许爱军[1] 曹菲[1] 田玉科[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院麻醉学教研室,武汉430030

出　　处：《中国疼痛医学杂志》2008年第3期163-166,共4页Chinese Journal of Pain Medicine

基　　金：国家自然科学基金资助课题(30570264)

摘　　要：目的:建立长春新碱诱发外周神经病理性疼痛大鼠模型。方法:雄性SD大鼠,隔日尾静脉注射长春新碱,共注射五次,造成外周神经病理性疼痛模型。测定2g和12g力度机械刺激引起的缩爪反应观察机械性痛觉超敏,辐射热测痛仪测量热痛觉过敏,同时行组织切片光镜和电镜下观察坐骨神经结构的破坏情况。结果:100μg/kg和150μg/kg组大鼠在第二次注射长春新碱后,开始出现机械性痛觉超敏和痛觉过敏,150μg/kg组大鼠有热痛觉过敏表现;组织形态学研究显示坐骨神经退行性变,轴突变性。结论:从疼痛行为学、组织学研究的结果,表明长春新碱诱发外周神经痛大鼠模型建立成功。Objective： To establish a rat model of vincristine-induced peripheral neuropathic pain. Methods ： Vincristine was administered intravenously every 2 days until five injections had been given in Sprague-Dawley rats. Mechanical allodynia and heat hyperalgesia were measured by using touch stimulator and radiant heat stimulator. The modification of structures in sciatic nerve was detected by histology. Results： Rats displayed the gradual development of mechanical allodynia and hyperalgesia , from the second injection of vincristine in the 100 μg/kg and 150μg/kg groups. The sciatic nerve in vincristine-treated rats degenerated was observed under electron microscopy. Conclusions： Results from the studies on behavioral tests and histology suggest that this model of vincristine-induced peripheral neuropathic pain has been copied well , and it will provide a useful animal model.

关 键 词：长春新碱 痛觉过敏 外周神经病理痛 

分 类 号：R402[医药卫生—临床医学]