高温胁迫下谷胱甘肽对离体玉米叶片的保护效应  

Protective Effects of Gluathione on Separated Leaves of Maize under High Temperature Stress

作　　者：陈大清[1] 王健[1] 

机构地区：[1]湖北农学院农学系,广西中医学院药学系

出　　处：《湖北农学院学报》1997年第4期254-256,共3页Journal of Hubei Agricultural College

基　　金：湖北省教委科研基金

摘　　要：室内培养玉米“掖单５１”幼苗至一叶一心，选择大小一致的展开叶片，切取中部２ｃｍ长叶段为试材，分别悬浮培养于３种不同处理条件的培养皿中：Ⅰ（ＣＫ）—蒸馏水＋室温２５℃；Ⅱ—蒸馏水＋４０℃；Ⅲ—ＧＳＨ（还原型谷胱甘肽）（１０μｍｏｌ／Ｌ）＋４０℃；于０ｈ，２ｈ，４ｈ，６ｈ进行有关生理指标的测定。结果表明：用１０μｍｏｌ／Ｌ的ＧＳＨ浸泡离体玉米叶片能减少高温胁迫下叶绿素和可溶性蛋白质含量的降低，但对ＰＯＤ活性有一定抑制作用，提示ＧＳＨ对高温胁迫下的离体玉米叶片有一定保护效应。Seedlings of maize variety (yedan 51) were cultivated in the room. When seedlings grew upon the period of one developed and a little new leaf. Some identical leaves were selected and cutted from middle parts in 2 cm as the materals. These materials were incubated in suspension in dishs at three different treatment. Ⅰ(CK)—distilled water+indoor temperature (25 ℃); Ⅱ—distilled water+40 ℃; Ⅲ—GSH (10 μmol/L)+40 ℃. Some physiological indices were determinated in the treatment time of 0 h, 2 h, 4 h, 6 h. The results shown that using solution of 10 μmol/L GSH to incubate the separated leaves of maize could reduce the falling of chlorophyll and soluble protein content. But the activity of POD was restrained in some degree. These suggested that GSH had some protective effects on separated leaves of maize under high temperature stress.

关 键 词：玉米 GSH 高温胁迫 离体叶片 保护效应 

分 类 号：S513.01[农业科学—作物学]