Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy  

作　　者：FAN XiaoJun ZHENG Bo FU YueJun SUN Yi LIANG AiHua 

机构地区：[1]Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China

出　　处：《Chinese Science Bulletin》2008年第12期1855-1860,共6页

基　　金：the National Natural Science Foundation of China (Grant Nos.30670282 and 30700534);the Department of Science and Technology of Shanxi Province

摘　　要：An Buthus martensii Karsch Insect Toxin (BmK IT ) gene was inserted into the genome of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) to construct a recombinant baculovirus, AcMNPV-BmK IT. The expression of BmK IT was confirmed using RT-PCR, dot blot and SDS-PAGE analysis. Dose-lethal time responses to Spodoptera exigua larvae were compared between wild-type baculovirus AcMNPV and recombinant virus AcMNPV-BmK IT. At the concentration of 1×107 PIBs/mL, the median lethal time of recombinant baculovirus (LT50 = 73.6h) on third instar S. exigua larvae showed an improvement of 13.2% over the efficacy of wild type virus (LT50 = 84.8h) during a 192h in-fection.An Buthus martensii Karsch Insect Toxin （BmK IT ） gene was inserted into the genome of Autographa californica multicapsid nucleopolyhedrovirus （AcMNPV） to construct a recombinant baculovirus, AcMNPV-BmK IT. The expression of BmK IT was confirmed using RT-PCR, dot blot and SDS-PAGE analysis. Dose-lethal time responses to Spodoptera exigua larvae were compared between wild-type baculovirus AcMNPV and recombinant virus AcMNPV-BmK IT. At the concentration of 1 × 10^7 PIBs/mL, the median lethal time of recombinant baculovirus （LT50 = 73.6 h） on third instar S. exigua larvae showed an improvement of 13.2% over the efficacy of wild type virus （LT50 = 84.8 h） during a 192 h infection.

关 键 词：杀虫剂 神经毒素 生物测定 基因组 

分 类 号：TQ453[化学工程—农药化工]