DNA methylation status of H19 and Xist genes in lungs of somatic cell nuclear transfer bovines  

作　　者：CHEN Jie LI DongJie LIU YanQin ZHANG Cui DAI YunPing LI ShiJie LI Ning 

机构地区：[1]College of Life Science, Hebei Agriculture University, Baoding 071001, China [2]Slate Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China [3]College of Life Science and Life Engineering, Hebei Science and Technology University, Shijiazhuang 050018, China

出　　处：《Chinese Science Bulletin》2008年第13期1996-2001,共6页

基　　金：the National High Technology Research and Development Program of China (Grant No.2001AA213091) ;Natural Science Foundation of Hebei Prov-ince (Grant No.C2006001032)

摘　　要：In somatic cell nuclear transfer (SCNT) technologies,the donor cell’s nuclei need to be epigenetically reprogrammed for embryonic development. The incomplete reprogramming of donor cell nuclei has been implicated as a primary reason for the low efficiency of SCNT. DNA methylation is a major epige-netic modification of the genome that regulates crucial aspects of genome function,including estab-lishment of genomic imprinting. In order to make sure whether the DNA methylation reprogramming is efficient in SCNT animals,we analyzed the DNA methylation status of two imprinting genes,H19 and Xist,in lungs of deceased SCNT bovines that died within 48 h of birth using bisulfite sequencing analysis. Our findings demonstrated that cloned bovines showed significantly lower DNA methylation of H19 than controls (P<0.05),and three tested CpGs sites (1,2,3) exhibited unmethylation in one cloned bovine (9C3); however,Xist showed similar DNA methylation levels between clones and con-trols,and both showed hypermethylation (96.11% and 86.67%).In somatic cell nuclear transfer （SCNT） technologies, the donor cell＇s nuclei need to be epigenetically reprogrsmmed for embryonic development. The incomplete reprogramming of donor cell nuclei has been Implicated as s primary reason for the low efficiency of SCNT. DNA methylstion is s major epigenetic modification of the genome that regulates crucial aspects of genome function, including establishment of genomic imprinting. In order to make sure whether the DNA methylstion reprogramming is efficient in SCNT animals, we analyzed the DNA methylstion status of two imprinting genes, H19 and Xist, in lungs of deceased SCNT bovines that died within 48 h of birth using bisulfite sequencing analysis. Our findings demonstrated that cloned bovines showed significantly lower DNA methylstion of H19 than controls （P〈0.05）, and three tested CpGs sites （1, 2, 3） exhibited unmethylstion in one cloned bovine （9C3）; however, Xist showed similar DNA methylation levels between clones and controis, and both showed hypermethylstion （96.11% and 86.67%）.

关 键 词：DNA甲基化 躯体细胞 核转移因子 牛 动物实验 

分 类 号：Q78[生物学—分子生物学]