骨骼肌损伤后修复过程中机械生长因子作用研究  

Research on Function of Mechano Growth Factor Following Skeletal Muscles Contusion

作　　者：肖卫华[1] 陆耀飞[2] 

机构地区：[1]湘南学院体育系,湖南郴州423000 [2]上海体育学院运动科学学院,上海200438

出　　处：《体育科学》2008年第6期34-38,共5页China Sport Science

摘　　要：目的:通过观察小鼠骨骼肌损伤后修复过程中机械生长因子(MGF)、M-cad基因表达,探讨MGF在伤后修复过程中所起作用;方法:健康雄性C57BL/6小鼠49只,按体重随机分为7组。其中1组不做任何处理作为对照组(C组),另6组均于同一天损伤,即损伤组(injury,1组)。小鼠腓肠肌钝挫伤后不同时期处死取材,对MGF、M-cad基因表达水平做实时定量RT-PCR分析;结果:骨骼肌损伤后1天MGF表达水平即显著升高(P<0.01),第4、第7天持续升高,第7天达峰值,第11天仍显著高于对照组。非损伤腿中MGF表达无显著变化。M-cad在非损伤腿中的表达与损伤腿相似,均在第7天表达达峰值(P<0.01);结论:MGF可能是激活肌卫星细胞的因子之一,但非惟一因子。MGF也可能与多肽合成有关。The purpose of this study was to analyze the MGF＇S function in the recover period after muscle injury by investigating the expression of MGF and M-cad. Mice were killed in different time after gastrocnemius injury and MGF and M-cad gene used for RT-PCR quantitative analysis. The result shows that there is a significant increase in MGF mRNA levels after 1 day （P〈0.01） ,which continued increase at 4 and 7 days following injury. It peaks at 7 days, however, MGF＇ S expression still significant higher than that of control group at 11 days. Compared with the control group, non-injury muscle＇s MGF mRNA levels have no significant difference. Similar to the injury muscle, M-cad rnRNA levels in contralateral non-injury muscle seemed to peak at 7 days（P〈0.01）. It concludes that MGF may be one but not only one factor which can active the rest muscle satellite cells. It may have some relationships with peptide synthesis.

关 键 词：骨骼肌 损伤 机械生长因子 胰岛素样生长因子-I 肌卫星细胞 M-钙粘蛋白 鼠 动物实验 

分 类 号：G804.5[文化科学—运动人体科学]