6种郁金类药用植物的同工酶研究  

Study on isozymes in six species of Curcuma

作　　者：汤加勇[1] 李青苗[2] 杨瑞武[1] 廖进秋[1] 周永红[3] 

机构地区：[1]四川农业大学生命科学与理学院,四川雅安625014 [2]四川省中医药科学院,四川成都610041 [3]四川农业大学小麦研究所,四川都江堰611830

出　　处：《中国中药杂志》2008年第12期1381-1386,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30670150);教育部长江学者和创新团队发展计划(IRT0453)

摘　　要：目的:探讨姜黄属Curcuma 6种郁金类药用植物39份材料间的亲缘关系。方法:采用聚丙烯酰胺凝胶电泳技术(PAGE),作过氧化物酶(POD)和酯酶(EST)同工酶分析,并用NTSYSpc 2.1软件对谱带作聚类分析。结果:在EST同工酶分析中,蓬莪术C.phaeocaulis、温郁金C.wenyujin、广西莪术C.kwangsiensis、川黄姜C.chuan- huangjiang有它们自己的特征谱带,而川郁金C.sichuanensis和姜黄C.longa有着共同的特征谱带;在POD同工酶分析中,只有蓬莪术和广西莪术有自己的特征谱带。结论:同种植物不同材料间亲缘关系的远近与地理来源关系不大;姜黄和川郁金亲缘关系很近,不能通过同工酶将它们区别开来。Objective： To explore genetic relationships of the 39 materials in six species of Curcuma. Method： The peroxidase isozyme （POD） and esterase isozyme （EST） were studied using vertical slab polyacrylamide gel electrophoresis （PAGE） technique, and the zymograms were analyzed using the software of NTSYSpc2.1. Result： The interspecific zymogramatic differences were obvious. Each species possessed its own specific zymogram distinguishing form the others. In the analysis of EST isozyme, C. phaeocaulis, C. wenyujin, C. kwangsiensis and C. chuanhuangjiang had their own specific zymogram. In the analysis of POD isozyme, just C. phaeocaulis and C. kwangsiensis had their specific zymogram. Conclusion： The genetic relationships are not associated with the geographical distributions and the genetic relationship between C. longa and C. sichuanensis are very close.

关 键 词：姜黄属 郁金 过氧化物酶 酯酶 聚类分析 

分 类 号：R282.5[医药卫生—中药学]