栽培黄芩和野生黄芩化学成分比较研究  

Comparative study on chemcial composition between cultivated and wild Radix Scutellariae

作　　者：李韦[1] 李化[1] 杨滨[1] 冯学锋[1] 格小光[1] 刘锦云[2] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]北京中医药大学,北京100102

出　　处：《中国中药杂志》2008年第12期1425-1429,共5页China Journal of Chinese Materia Medica

基　　金：科研院所社会公益研究专项(2005DIA1J172)

摘　　要：目的:比较栽培黄芩和野生黄芩化学成分的差异。方法:收集不同产地栽培和野生黄芩共30余批,采用高效液相色谱法建立黄芩药材的色谱指纹图谱,并对黄芩苷、黄芩素和汉黄芩素进行含量测定;采用《中国药典》方法对黄芩的浸出物进行含量测定。结果:河北和内蒙古产的野生黄芩的液相指纹图谱色谱峰数较栽培品多;在95%的置信区间内,栽培黄芩含黄芩苷(15.89±1.52)%,黄芩素为(1.04±0.26)%,汉黄芩紊(0.27±0.07)%;野生黄芩含黄芩苷(11.93±1.62)%,黄芩素(1.03±0.26)%,汉黄芩素(0.27±0.06)%;栽培黄芩的浸出物为(52.07±3.05)%,野生黄芩(41.21±2.86)%。结论:栽培黄芩和野生黄芩的指纹图谱略有差异;栽培黄芩中黄芩苷和浸出物的含量高于野生黄芩。Objective： To do some comparative study on chemical composition between the cultivated and wild samples of Radix Scutellariae. Method： Thirty three samples of Radix Scutellariae were collected from different habitats. An HPLC method for fingerprint identification and simultaneous determination of baicalin, baicalein and wogonin was established, and the content of ethanolic extractive was measured according to the method in Chinese Pharmacopoeia. Result： The number of chromatographic peaks in fingerprint of wild sample was more than that of cultivated sample in Hebei and Inner Mongolia. In 95% confidence intervals, the contents of baicalin, baicalein and wogonin were （ 15.89 ± 1.52 ） % , （ 1.04 ± 0. 26 ） % and （ 0. 27 ± 0. 07 ） %, respectively in the cultivated samples and （ 11.93 ± 1.62 ） % , （ 1.03 ± 0. 26 ） % and （ 0. 27 ± 0. 06 ） % , respectively in wild samples. The content of ethanolic extractive was 52. 07 ± 3.05% in the cultivated and （41.21 ± 2. 86）% in the wild within 95% confidence interval. Conclusion： Slight difference between the fingerprint of the cultivated and wild samples was found, and the contents of baicalin and ethanolic extraction in the cultivated were higher than those in the wild.

关 键 词：黄芩 栽培品和野生品 高效液相色谱法 浸出物含量 

分 类 号：R284.1[医药卫生—中药学]