Cedemex对吗啡戒断大鼠不同脑区cAMP和cGMP含量的影响  

Effect of Cedemex on cAMP and cGMP levels of different brain areas in morphine withdrawal rats

作　　者：谢海源[1] 赖术[1] 黄建春[1] 蒋伟哲[1] 郭松超[1] 黄仁彬[1] 阮富桥 范金敏 梁越高 陈亭平 

机构地区：[1]广西医科大学药理学教研室,广西南宁530021 [2]越南重大疾病研究院,越南河内

出　　处：《中国中药杂志》2008年第12期1439-1443,共5页China Journal of Chinese Materia Medica

基　　金：中越政府间科技合作联委会第五次会议长期合作研究开发项目(5-303J);广西自然科学基金项目(0575066)

摘　　要：目的:观察Cedemex对纳洛酮催促下的戒断大鼠不同脑区环磷腺苷(cAMP)和环磷鸟苷(cGMP)含量的影响。方法:设空白、正常Cedemex、停用吗啡、续用吗啡、Gedemex 8,4,2 g·kg^(-1)·d^(-1)、益安口服液组8个实验组,每组10只大鼠。以剂量递增法连续皮下注射吗啡7 d建立吗啡依赖大鼠模型,采用放射免疫方法测定中脑腹侧被盖区(VTA)、皮层和海马的cAMP和cGMP含量。结果:Cedemex对大鼠吗啡戒断症状具有明显的抑制作用(P<0.05);与空白组相比较,续用吗啡组能够使依赖大鼠的VTA、皮层和海马内cAMP含量显著增高,cGMP含量显著下降(P<0.05);短期使用Cedemex可使吗啡戒断大鼠VTA、皮层和海马内cAMP含量明显下降,cGMP明显增高(P<0.05)。结论:Cedemex可显著抑制大鼠吗啡戒断反应,其可能的机制与Cedemex能调节中枢cAMP和cGMP含量有关。Objective： To investigate the effect of Cedemex on cAMP and cGMP contents in different brain regions in morphine withdrawal rats precipitated by naloxone. Method： A physical morphine dependent model of rats was established by subcutaneous injection of morphine in gradually increasing dosage within 7 days. cAMP and cGMP contents of VTA, cortex and hippocampus of the rat brains were determined by radioimmunoassay. Result： The morphine withdrawal symptoms of rats were relieved significantly by Cedemex. Compared with the controls, cAMP content in the region of VTA, cortex and hippocampus of the morphine dependent rats were significantly higher （ P 〈 0. 05 ）, while cGMP contents in those regions were significantly lower （ P 〈 0. 05 ）. cAMP contents in the area of VTA, cortex and hippocampus of the morphine dependent rats were significantly reduced, while cGMP contents were significantly increased by ig Cedemex. Conclusion： Cedemex may significantly attenuate the morphine withdrawal symptoms in rats. The mechanism of this effect may be related to adjusting the contents of cAMP and eGMP in some brain regions.

关 键 词：Cedemex 吗啡依赖 戒断症状 环磷腺苷 环磷鸟苷 

分 类 号：R285.5[医药卫生—中药学]