羊泰勒虫吉林省分离株18SrRNA基因的克隆和序列分析  

Cloning and sequence analysis of Jilin isolated 18S rRNA gene of Theileria sp

作　　者：田万年[1] 薛书江[1] 刘冰[1] 丁德[1] 张守发[1] 

机构地区：[1]延边大学农学院动物医学系,吉林龙井133400

出　　处：《中国兽医杂志》2008年第6期8-10,共3页Chinese Journal of Veterinary Medicine

摘　　要：为分析羊泰勒虫吉林省分离株的18S rRNA基因序列,根据羊泰勒虫18S rRNA基因的保守区序列设计1对引物,对吉林省的绵羊血液基因组DNA进行PCR扩增,结果扩增出长度为459 bp的基因片段,并成功地将该基因克隆到pMD18-T载体。将经纯化、筛选及酶切鉴定和PCR鉴定为阳性的重组质粒进行测序,与已发表的羊泰勒虫基因序列进行比较。序列分析显示,羊泰勒虫吉林省分离株与羊泰勒虫China 1的同源性为100%。To analyse the 18S rRNA nucleotide sequence of Theileria sp in Jilin,a pair of primers were designed according to the 18S ribosomal RNA（18S rRNA） gene sequences pulished in the GenBank.The 18S rRNA gene of the Jilin isolate was amplified by PCR and cloned into pMD18-T vector.The recombinant plasmid were transformed into E.coli DH5α and identified by HindⅢ digestion and PCR.The fragment was sequenced and the sequence of nucleotides was analysed with published Theileria sp 18S rRNA gene sequence.Results showed that the fragment size is 459 bp and the homology of nucleotide sequence between Jilin isolate and China 1 strain was 100%.

关 键 词：羊泰勒虫 18S RRNA基因 PCR 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]