秦岭链霉菌的原生质体再生与诱变育种  

Protoplast Regeneration and Mutagenesis Breeding of Streptomyces qinlingensis sp. nov.

作　　者：卞小莹[1] 吴文君[1] 王群利[1] 方丽萍[1] 

机构地区：[1]西北农林科技大学农药研究所,杨凌712100

出　　处：《微生物学通报》2008年第6期929-933,共5页Microbiology China

基　　金：国家"973项目"资助(No.2003CB114404)

摘　　要：在秦岭链霉菌(Streptomyces qinlingensis sp.nov.)的菌种改良中,应用原生质体再生并结合物理化学诱变能够得到产量较高、稳定性较好的菌株。筛选实验表明:秦岭链霉菌原生质体再生菌株R-72、诱变菌株NTG-1和H30-7对枯草芽孢杆菌的抗菌活性均提高了20%以上,并且连续培养10代,其遗传性状均比较稳定。进一步的生测实验表明菌株R-72、NTG-1和H30-7对5种病原细菌和5种植物病原真菌的抗菌活性相比原始菌株有显著提高。To improve the antibiotics production of Streptomyces qinlingensis sp. nov., protoplast regeneration combined with physical and chemical mutagenesis was used to selected high-yielding strains. The results showed that the antibacterial activities of strain R-72 from protoplast regeneration and NTG-1, H30-7 from protoplast mutagenesis against Bacillus subtilis were more than 20% higher than that of the original strain, and the heredity characters of those strains were stable in successive ten generations. The further bioassay experiments exhibited that the fungicidal and antibacterial activities of the fermentation broth from R-72, NTG- 1 and H30-7 were remarkable increased comparing with that of the starting strain.

关 键 词：秦岭链霉菌 原生质体再生 原生质体诱变 筛选 

分 类 号：Q93[生物学—微生物学]