细胞色素c/L-半胱氨酸修饰金电极检测亚硝酸根  

Voltammetric Determination of Nitrite Radical Using the Modified Electrode L-Cys/Au Immobilized with Cytochrome c

作　　者：李梦瑶[1] 孔令蜜[1] 朱佩思[1] 彭斌[1] 黄杉生[1] 袁寅祥[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《理化检验（化学分册）》2008年第6期495-498,510,共5页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家高技术研究发展计划(813)项目(06Z4);上海市教委项目(06ZZ17)

摘　　要：采用循环伏安法、交流阻抗法研究了组装在L-半胱氨酸（L-Cys）修饰金电极上的细胞色素c（Cytc）电化学行为；采用电化学方法以及紫外一可见光谱对电极进行表征。结果表明：通过静电吸附作用组装在L-Cys修饰金电极上的细胞色素c保持了良好的生物和电化学活性，用Cytc／L-Cys修饰金电极检测亚硝酸根，响应电流与亚硝酸根浓度在5．0×10^-6～4．5×10^-4mol·L^-1范围内呈线性关系，线性回归方程为Ip=0．031c＋8．165×10^-6，相关系数为0．9995，检出限（S／N=3）为1．5×10^-7mol·L^-1。电极用于模拟样品及咸菜样品中亚硝酸根的测定，回收率为89．0％～116．0％。Electrochemical behavior of cytochrome c immobilized on the modified electrode of L-cystein/Au （abbreviated as Cyt. c/L-Cys/Au） was studied by cyclic voltammetry and alternating impedance, and the modified electrode was characterized by electrochemical methods and UV-VIS spectrophotometry. As shown by experimental results, cytochrome c, immobilized electrostatically on the L-cysteine/Au electrode, behaved to keep its biological and electrochemical activity in a good state. The modified electrode Cyt. c/L-Cys/Au was used as working electrode in the voltammetric determination of nitrite radical, and linear relationship between the values of response peak current （Ip） and concentration of NO2^- ion was obtained in the range from 5.0 × 10^-6 to 4. 5 × 10^-4mol · L^-1 in respective to the linear regression equation of Ip=0. 031 c＋8. 165× 10^-6 （r=0. 999 5）. Detection limit （S/N=3） of the method was found to be 1.5× 10^-7mol · L^-1. Simulated samples and samples of pickles were analyzed by the proposed method, giving values of recovery in the range of 89.0%-116.0% ：

关 键 词：伏安法 修饰金电极 L-半胱氨酸 细胞色素C 亚硝酸根 

分 类 号：O646[理学—物理化学]