脑出血大鼠心肌组织TNF-α蛋白表达的变化及扶正护脑胶囊的干预作用  

The Effect of Fuzhenghunao Capsule on the Protein Expression of TNF-α of Myocardial Tissues in Rats with Intracerebral Hemorrhage

作　　者：赵彦青[1] 王伟民[1] 申亚晖[1] 宋文翔[1] 

机构地区：[1]河南省中医院,郑州450002

出　　处：《中国中医急症》2008年第6期809-810,共2页Journal of Emergency in Traditional Chinese Medicine

基　　金：河南中医学院博士科研启动基金资助项目(No.03301048)

摘　　要：目的探讨脑出血大鼠心肌组织TNF-α蛋白表达的变化及扶正护脑胶囊的干预作用。方法动物随机分为假手术组、模型组、扶正护脑组(治疗组)和安宫牛黄组(对照组),分别于术后6、24、72三个时相点取材,采用免疫组织化学法测定心肌组织TNF-α蛋白表达,通过图像分析测定IOD值;并与血清CK-MB、cTnI含量作相关性分析。结果TNF-α阳性颗粒主要在心内膜下的心肌细胞和血管内皮细胞的胞浆中表达。结论炎性因子TNF-α参与了脑出血诱发心肌损伤的机制,扶正护脑胶囊通过抑制TNF-α蛋白表达减轻脑出血后心肌的损伤。Objective： To observe the effect of Fuzhenghunao capsule on TNF - α protein expression of myocardial tissues in rats with intracerebral hemorrhage. Methods： 72 rats were randomly divided into 4 groups： sham group, model group, Fuzhenghunao group and Angongniuhuang group. The material was selected at 6, 24, 72h after operation. The protein expression of TNF -α in rat myocardial tissues was detected by the immunohistochemistry method, and measurd the integrated optical density（IOD） with image analysis. Explored the correction between the IOD of TNF -α and the content of CK - MB and cTnI. Results： The positive paritive particles of TNF -α was in the cytosol of myocardial cell and vascular endotheliocyte near to the endocardium. Conclusion： Inflammatory response attends the myocardial damage. Fuzhenghunao capsule can protect the myocardial tissues by inhibiting the protein expression of TNF -α.

关 键 词：脑出血 心肌损伤 肿瘤坏死因子-Α 扶正护脑胶囊 

分 类 号：R285.5[医药卫生—中药学]