正交分析法优化开郁胶囊中芍药苷的提取工艺  

Optimizes the Extraction of Paeoniflorin in Kaiyu Capsule by Orthogonal Analysis

作　　者：刘莹[1] 王文[1] 魏海峰[1] 刘茨[1] 艾厚喜[1] 李雅莉[1] 李林[1] 

机构地区：[1]首都医科大学宣武医院药物研究室,教育部神经变性病学重点实验室,北京100053

出　　处：《中国药师》2008年第6期620-621,共2页China Pharmacist

基　　金：国家重点基础研究973计划(No.2003CB517104);国家自然科学基金(No.30472184;90709011);北京市自然科学基金(No.7050001)

摘　　要：目的:优选开郁胶囊中有效成分芍药苷的醇提工艺条件。方法:采用正交试验方法,对影响芍药苷醇提工艺的药材煎煮次数、乙醇用量、煎煮时间、醇提浓度等因素和不同水平进行考核。应用高效液相色谱法(HPLC)测定不同条件下芍药苷的转移率。结果:最优工艺为采用12倍量的60%乙醇加热回流提取3次,每次1 h。结论:以上条件为开郁胶囊醇提的最佳工艺,可为开郁胶囊的工业化生产提供理论依据。Objective： To establish an extraction method of paeoniflorin from Kaiyu capsule containing Radix Paeoniae Alba for the treatment of depression. Method ： The orthogonal test was used to optimize the extracting conditions. Paeoniflorin in Radix Paeoniae Alba was taken as an evaluating marker and was determined by HPLC. The effects of different extraction methods on the extraction efficiency of the paeoniflorin were investigated. Result： The optimum extraction condition were refluxing extraction with twelve times amount of 60% alcohol for 3 times, 1 hour each time. Conclusion： Above selected conditions may be the best way to extract paeoniflorin in Kaiyu capsule.

关 键 词：白芍 芍药苷 正交实验 提取工艺 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]