Heredity Analysis and Gene Mapping of Bruchid Resistance of a MungbeanCultivar V2709  

作　　者：SUN Lei CHENG Xu-zhen WANG Su-hua WANG Li-xia LIU Chang-you MEI Li XU Ning 

机构地区：[1]Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

出　　处：《Agricultural Sciences in China》2008年第6期672-677,共6页中国农业科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(30170635);Crop Germplasm Protection,China(NB04-22-12);National Facilities and Information Infrastructure for Scinece and Technology,China(2004DKA30380-08,National Key Technology Support Plan,China(2006BAD02B08),Science and Technology of Agricultural Vocation,China(nyhyzx07-017).

摘　　要：Bruchid is a serious insect pest of mungbean [Vigna radiata （L.） Wilczek] and can inflict serious loss. A resistant variety from India, V2709, was crossed with a susceptible variety, Zhonglti 1, from the World Vegetable Center, Asian Vegetable Research and Development Center （AVRDC）. Segregation of the F2, BC1F1, and F3 populations showed that bruchid resistance of V2709 is controlled by a single dominant locus. To find molecular marker linked with the resistant locus, 63 randomly amplified polymorphic DNA markers and 113 sets of SSR/STS primers were used in a bulked segregant analysis. Two of the markers, OPC-06 and STSbr2, were found to be linked with the locus （named as Br2）. Further analysis suggested that the genetic distances between these two markers and Br2 were 11.0 and 5.8 cM, respectively.Bruchid is a serious insect pest of mungbean [Vigna radiata （L.） Wilczek] and can inflict serious loss. A resistant variety from India, V2709, was crossed with a susceptible variety, Zhonglti 1, from the World Vegetable Center, Asian Vegetable Research and Development Center （AVRDC）. Segregation of the F2, BC1F1, and F3 populations showed that bruchid resistance of V2709 is controlled by a single dominant locus. To find molecular marker linked with the resistant locus, 63 randomly amplified polymorphic DNA markers and 113 sets of SSR/STS primers were used in a bulked segregant analysis. Two of the markers, OPC-06 and STSbr2, were found to be linked with the locus （named as Br2）. Further analysis suggested that the genetic distances between these two markers and Br2 were 11.0 and 5.8 cM, respectively.

关 键 词：mungbean V2709 bruchid resistance heredity analysis STS RAPD 

分 类 号：S643[农业科学—蔬菜学]