濒危植物长序榆的FTIR分析及与土壤元素氮的相关性研究  

FTIR Spectra of Endangered Plants Ulmus Elongata and Its Correlation to Soil Nitrogen

作　　者：张志祥[1] 刘鹏[1] 康华靖[1] 廖承川 潘成椿 李成惠 

机构地区：[1]浙江师范大学植物学实验室,浙江金华321004 [2]九龙山国家级自然保护区管理局,浙江遂昌323300

出　　处：《光谱学与光谱分析》2008年第6期1255-1259,共5页Spectroscopy and Spectral Analysis

基　　金：浙江省自然科学基金项目(399277);浙江省新苗人才计划项目(2007G60G2030026);金华市科技项目(2005-1-318)资助

摘　　要：应用傅里叶变换红外光谱(FTIR)法测定了8个样地长序榆的根、茎、皮、叶的红外光谱,并对这些红外光谱与土壤元素氮进行了相关分析。结果表明,根、茎、皮、叶的红外光谱存在一定差异,其组织器官化学成分含量和土壤氮元素之间存在不同程度的相关关系,茎中化学成分含量和全氮都呈显著正相关(p<0.05);皮在3 365 cm-1波数时的化学成分含量和全氮呈显著正相关,根和叶的化学成分含量与全氮呈低度相关。土壤速效氮和长序榆根、茎、皮、叶的化学成分含量之间也存在一定程度的相关性,但相关性比全氮弱的多,均未达到统计学意义上的"显著水平"。说明土壤全氮的变化对长序榆植物的化学成分具有一定的影响,速效氮的影响则相对较低。Ulmus elongata, an endemic species in China, is one of the grade Ⅱ national key conservation rare and endangered plants. The spectra of root, stem, skin and leaf of Ulmus elongata sampled from eight different sites were determined by Fourier transform infrared （FTIR） spectrometry with OMNI-sampler directly, fast and accurately. A positioning technology of OMNIC E. S. P. 5.1 intelligent software and ATR correction was used. The background was scanned before the determination of every example. The peak value and absorbance were ascertained using a method of baseline correction in infrared spectra, and then the relativity between absorption peaks of the spectra and the soil nitrogen was analyzed. Results from the comparison of the spectra showed some differences in their FTIR spectra among root, stem, skin and leaf of Ulmus elongata from the same plant. The coefficients of correlation between chemical composition of this four different organs of Ulmus elongata and soil nitrogen were positive in different degrees. There was the significantly positive correlation between chemical composition of stem and total nitrogen （p〈0. 05）. When the wave-number was 3 365 cm^-1 , there was a significantly positive correlation between chemical composition of skin and total nitrogen, and a low correlation between root and leave chemical composition and total nitrogen. There was also a certain extent correlation between chemical composition of this four different organs of Ulmus elongata and soil available nitrogen, but the coefficients of correlation was smaller, and the level of the statistic significance was not significant （p〉0. 05）. It was showed that the change in soil total nitrogen has some influence on chemical composition of different organs of Ulmus elangata, but the degree of available nitrogen was very smaller. The linear correlation between soil total nitrogen and organs chemical composition of Ulmus elongate, not only provided the theoretic basis for plant nutriology and nutrient ecology of Ulmus elon

关 键 词：傅里叶变换红外光谱 全氮 速效氮 相关性 化学成分 长序榆 

分 类 号：O657.3[理学—分析化学]