小麦1BL/1RS易位系的检测  

Detection of 1BL/ 1RS Translocation in Wheat

作　　者：冯国华[1] 王晓军[1] 刘东涛[1] 王静[1] 张会云[1] 赵军海[1] 陈荣振[1] 

机构地区：[1]徐州市农业科学院,江苏徐州221121

出　　处：《西北农业学报》2008年第3期81-85,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家863计划项目(2006AA100102);国家科技支撑计划项目(2006BAD01A02-18);国家农业科技成果转化项目(2006GB2C100103);公益性行业(农业)科研专项项目(nyhyzx07-002);江苏省高技术研究项目(BG2006305)

摘　　要：1BL/1RS小麦-黑麦易位对小麦的面包烘烤品质有很强的负面影响,快速准确地鉴定1BL/1RS易位对小麦的品质育种有重要意义。本文通过黑麦碱蛋白的SDS-PAGE电泳,分析比较了4个1BL/1RS小麦-黑麦易位相关基因的分子标记的特异性。结果表明,在供试的51份材料中,低分子量麦谷蛋白Glu-B3基因和黑麦碱蛋白Sec基因特异标记的PCR检测结果与蛋白电泳结果一致。同时也验证了一个多重PCR分子标记(Glu-B3引物和Sec-P1,P2引物复合)的可靠性。该共显性多重PCR分子标记不但能鉴定出1BL/1RS易位系,而且能鉴定出该位点的杂合情况,是适用于优质强筋小麦标记辅助选择的理想标记。The 1BL/1RS wheat-rye translocation has great negative effects on wheat bread-making quality.So,it is very important to detect the 1BL/1RS translocation rapidly and exactly for the strong gluten wheat breeding.Four specific PCR markers related to the 1BL/1RS wheat-rye translocation,including one marker of low molecular weight glutenin gene Glu-B3 and three specific PCR markers of ω-secalin gene,were compared and verified by SDS-PAGE method in this paper.The PCR assay of the four markers is accordant well with the results of SDS-PAGE in the 51 analyzed varieties.And a co-dominant multiplex PCR,which composed of two primer pairs for Glu-B3 and Sec respectively, was validated in these varieties at the same time.It was confirmed to be a reliable and stable molecular marker for bread wheat breeding,with the advantage of could distinguish the heterozygous plants from the homozygous ones.

关 键 词：小麦 1BL/1RS易位 复合引物 

分 类 号：S512.1[农业科学—作物学]